| Efficient production of poly-gamma-glutamic acid by Bacillus subtilis ZJU-7. | |
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MedLine Citation:
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PMID: 16720907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A strain with high poly-gamma-glutamic acid (gamma-PGA) production was isolated from fermented bean curd, a traditional Chinese food. The strain was named Bacillus subtilis ZJU-7 according to 16s rDNA sequencing and its taxonomic characters. The culture conditions for gamma-PGA production were evaluated. The most suitable carbon and nitrogen sources were sucrose and tryptone, respectively. Exogenous L-glutamic acid was necessary for gamma-PGA production, and the production of gamma-PGA increased on the addition of L-glutamic acid to the medium. In the medium containing 60 g/L of sucrose, 60 g/L of tryptone, 80 g/L of L-glutamic acid, and 10 g/L of NaCl, the yield of gamma-PGA reached 54.4 g/L after cultivation at 37 degrees C for 24 h, which was the highest gamma-PGA production compared with values reported in the literature. The average molecular mass of gamma-PGA produced was about 1.24 x 106 Daltons. B. subtilis ZJU-7 is genetically stable and can synthesize levan instead of gamma-PGA without the addition of L-glutamic acid to the medium. |
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Authors:
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Feng Shi; Zhinan Xu; Peilin Cen |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 133 ISSN: 0273-2289 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-24 Completed Date: 2006-09-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 271-82 Citation Subset: IM |
Affiliation:
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Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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metabolism* Culture Media Fermentation* Glutamic Acid / pharmacology Industrial Microbiology Molecular Weight Peptones / pharmacology Polyglutamic Acid / biosynthesis* RNA, Ribosomal, 16S / genetics Sodium Chloride / pharmacology Soybeans / chemistry* Sucrose / pharmacology Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Peptones; 0/RNA, Ribosomal, 16S; 25513-46-6/Polyglutamic Acid; 56-86-0/Glutamic Acid; 57-50-1/Sucrose; 73049-73-7/tryptones; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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