| Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer. | |
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MedLine Citation:
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PMID: 20596920 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Omega-3(omega-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of omega-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene. |
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Authors:
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DengKe Pan; Li Zhang; YanRong Zhou; Chong Feng; Chuan Long; Xiao Liu; Rong Wan; Jian Zhang; AiXing Lin; EnQiu Dong; ShuChen Wang; HouGang Xu; HongXing Chen |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-07 |
Journal Detail:
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Title: Science China. Life sciences Volume: 53 ISSN: 1869-1889 ISO Abbreviation: Sci China Life Sci Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-07-02 Completed Date: 2010-11-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101529880 Medline TA: Sci China Life Sci Country: China |
Other Details:
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Languages: eng Pagination: 517-23 Citation Subset: IM |
Affiliation:
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Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. pandengke@yahoo.com.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified* China Diploidy Embryo Transfer Fatty Acid Desaturases / genetics* Fatty Acids, Omega-3 / genetics, metabolism Fibroblasts / metabolism Nuclear Transfer Techniques* Sus scrofa / genetics* Transfection |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Omega-3; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/omega-3 fatty acid desaturase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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