Document Detail


Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer.
MedLine Citation:
PMID:  20596920     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Omega-3(omega-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of omega-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene.
Authors:
DengKe Pan; Li Zhang; YanRong Zhou; Chong Feng; Chuan Long; Xiao Liu; Rong Wan; Jian Zhang; AiXing Lin; EnQiu Dong; ShuChen Wang; HouGang Xu; HongXing Chen
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-07
Journal Detail:
Title:  Science China. Life sciences     Volume:  53     ISSN:  1869-1889     ISO Abbreviation:  Sci China Life Sci     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-07-02     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101529880     Medline TA:  Sci China Life Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  517-23     Citation Subset:  IM    
Affiliation:
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. pandengke@yahoo.com.cn
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified*
China
Diploidy
Embryo Transfer
Fatty Acid Desaturases / genetics*
Fatty Acids, Omega-3 / genetics,  metabolism
Fibroblasts / metabolism
Nuclear Transfer Techniques*
Sus scrofa / genetics*
Transfection
Chemical
Reg. No./Substance:
0/Fatty Acids, Omega-3; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/omega-3 fatty acid desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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