Document Detail

Efficient production of (R)-3-hydroxycarboxylic acids by biotechnological conversion of polyhydroxyalkanoates and their purification.
MedLine Citation:
PMID:  17206818     Owner:  NLM     Status:  MEDLINE    
An efficient method to prepare enantiomerically pure (R)-3-hydroxycarboxylic acids from bacterial polyhydroxyalkanoates (PHAs) accumulated by Pseudomonas putida GPo1 is reported in this study. (R)-3-Hydroxycarboxylic acids from whole cells were obtained when conditions were provided to promote in vivo depolymerization of intracellular PHA. The monomers were secreted into the extracellular environment. They were separated and purified by acidic precipitation, preparative reversed-phase column chromatography, and subsequent solvent extraction. Eight (R)-3-hydroxycarboxylic acids were isolated: (R)-3-hydroxyoctanoic acid, (R)-3-hydroxyhexanoic acid, (R)-3-hydroxy-10-undecenoic acid, (R)-3-hydroxy-8-nonenoic acid, (R)-3-hydroxy-6-heptenoic acid, (R)-3-hydroxyundecanoic acid, (R)-3-hydroxynonanoic acid, and (R)-3-hydroxyheptanoic acid. The overall yield based on released monomers was around 78 wt % for (R)-3-hydroxyoctanoic acid. All obtained monomers had a purity of over 95 wt %. The physical properties of the purified monomers and their antimicrobial activities were also investigated.
Katinka Ruth; Andreas Grubelnik; René Hartmann; Thomas Egli; Manfred Zinn; Qun Ren
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomacromolecules     Volume:  8     ISSN:  1525-7797     ISO Abbreviation:  Biomacromolecules     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-08     Completed Date:  2007-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  279-86     Citation Subset:  IM    
Laboratories for Biomaterials, Swiss Federal Laboratories for Materials Testing and Research, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland.
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MeSH Terms
Alkanes / chemistry*
Anti-Infective Agents / chemistry
Biotechnology / methods*
Calorimetry, Differential Scanning / methods
Carbon / chemistry
Carboxylic Acids / chemistry*
Chromatography / methods
Chromatography, Gas / methods
Chromatography, Liquid / methods
Macromolecular Substances
Magnetic Resonance Spectroscopy
Models, Chemical
Molecular Conformation
Pseudomonas putida / metabolism
Solvents / chemistry
Reg. No./Substance:
0/Alkanes; 0/Anti-Infective Agents; 0/Carboxylic Acids; 0/Macromolecular Substances; 0/Solvents; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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