| Efficient production of 2-pyrone 4,6-dicarboxylic acid as a novel polymer-based material from protocatechuate by microbial function. | |
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MedLine Citation:
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PMID: 16322989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sphingomonas paucimobilis SYK-6, which can degrade various low molecular weight compounds derived from plant polyphenols such as lignin, lignan, and tannin, metabolizes these substances via 2-pyrone-4,6-dicarboxylic acid (PDC). We focused on this metabolic intermediate as a potential raw material for novel, bio-based polymers. We cloned the ligAB and ligC genes of SYK-6, which respectively encode protocatechuate 4,5-dioxygenase and 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase, into a broad host range plasmid vector, pKT230MC. The resulting plasmid, pDVABC, was introduced into the PpY1100 strain of Pseudomonas putida, and we found that PDC could be stably produced from protocatechuate and accumulated. In addition, we examined the efficiency of production of PDC from protocatechuate on a 5-L scale in a Luria-Bertani medium containing 100 mM glucose and determined that PDC was stably produced from protocatechuate to yield 10 g/L or more. |
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Authors:
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Yuichiro Otsuka; Masaya Nakamura; Kiyotaka Shigehara; Kosuke Sugimura; Eiji Masai; Seiji Ohara; Yoshihiro Katayama |
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Publication Detail:
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Type: Evaluation Studies; Journal Article Date: 2005-12-02 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 71 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-07-21 Completed Date: 2006-10-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 608-14 Citation Subset: IM |
Affiliation:
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Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism Biotechnology / methods Culture Media Dioxygenases / genetics, metabolism* Escherichia coli / enzymology, genetics, growth & development Hydroxybenzoic Acids / metabolism* Polymers / metabolism Pseudomonas putida / enzymology, genetics, growth & development Pyrones / metabolism* Sphingomonas / enzymology*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Culture Media; 0/Hydroxybenzoic Acids; 0/Polymers; 0/Pyrones; 72698-24-9/alpha-pyrone-4,6-dicarboxylic acid; 99-50-3/protocatechuic acid; EC 1.13.11.-/Dioxygenases; EC 1.13.11.8/protocatechuate 4,5-dioxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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