Document Detail


Efficient preparation of plant chromosomes for high-resolution scanning electron microscopy.
MedLine Citation:
PMID:  7981946     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A highly reproducible technique to prepare plant chromosomes for high-resolution field emission scanning electron microscopy is presented. The procedure allows the production of relatively high numbers of chromosome spreads that can be viewed at high resolution, showing structural details below 10 nm. This preparation technique is not restricted to metaphase chromosomes, but also allows the observation of plant chromosomes during all stages of the cell cycle.
Authors:
R Martin; W Busch; R G Herrmann; G Wanner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  2     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1995-01-05     Completed Date:  1995-01-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  411-5     Citation Subset:  IM    
Affiliation:
Botanisches Institut, Ludwig-Maximilians-Universität, Munich, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chromosomes / ultrastructure*
Hordeum / genetics*
Microscopy, Electron, Scanning
Specimen Handling

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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