Document Detail


Efficient preparation of liposomes encapsulating food materials using lecithins by a mechanochemical method.
MedLine Citation:
PMID:  17693697     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to evaluate to the feasibility of using lecithins for nanocapsules including functional food materials, liposomes were prepared from different commercially available lecithins (SLP-WHITE, SLP-PC70 and PL30S) by the Bangham method, and their physicochemical properties were examined by using a confocal laser scanning microscopy (CLSM) and the measurements of trapping efficiency. There was little difference in the trapping efficiency among the three types of liposomes. In all cases, the trapping efficiency clearly increased with an increase of the lecithin concentration up to 10 wt % , and the maximum efficiency reached at approximately 15%. CLSM observation showed the particle size of liposomes prepared from SLP-WHITE is significantly smaller than that prepared from other lecithins. In addition, liposomal solution prepared from SLP-WHITE remained well dispersed for at least 30 days, while two other liposomal solutions showed a phase separation due to aggregation and/or fusion of liposomes. These results indicated that SLP-WHITE is the most appropriate for the preparation of stable liposomes with well dispersed among the lecithins tested. SLP-WHITE liposomes were then prepared by the mechanochemical method using a homogenizer and microfluidizer, aiming at improving the preparation efficiency and liposome stability. The particle size of the prepared SLP-WHITE liposomes decreased with increasing inlet pressure and the number of processed cycles, and reached between 73 and 123 nm based on the measurement using dynamic light scattering. Moreover, freeze-fracture transmission electron microscopy revealed that the prepared liposomes are small unilamellar vesicles (SUV) with a diameter of approximately 100 nm. The extract of Curcuma longa Linn. (Ukon), which contains curcumins as a functional food material, was then subjected to the mechanochemical method with SLP-WHITE to give liposomes including the functional materials. Interestingly, the trapping efficiency of the liposomes for curcumins was found to reach over 85%. From these results, the present mechanochemical method is very likely to allow us to efficiently prepare stable and functional liposomes from the low-cost lecithin. The method may thus have a potential for manufacturing practical nanocapsules, which serves as a novel carrier of functional food materials.
Authors:
Makoto Takahashi; Kei-ichiro Inafuku; Takeshi Miyagi; Hirosuke Oku; Koji Wada; Tomohiro Imura; Dai Kitamoto
Related Documents :
21682147 - Validation and reproducibility of a semi-quantitative food frequency questionnaire for ...
21865567 - 20-year trends in dietary and meal behaviors were similar in u.s. children and adolesce...
24295677 - Amazonian palm oenocarpus bataua ("patawa"): chemical and biological antioxidant activi...
21696827 - Concentrations and seasonal variations of polybrominated diphenyl ethers (pbdes) in in-...
1798777 - The effects of chronic ventromedial hypothalamic stimulation on weight gain in rats.
11449267 - Discovery of 12 satellites of saturn exhibiting orbital clustering.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of oleo science     Volume:  56     ISSN:  1347-3352     ISO Abbreviation:  J Oleo Sci     Publication Date:  2006  
Date Detail:
Created Date:  2007-08-13     Completed Date:  2007-09-28     Revised Date:  2009-08-11    
Medline Journal Info:
Nlm Unique ID:  101175339     Medline TA:  J Oleo Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  35-42     Citation Subset:  IM    
Affiliation:
The United Graduate School of Agricultural Science, Kagoshima University, JAPAN.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chemistry, Pharmaceutical / methods*
Food*
Liposomes / chemical synthesis*
Mechanics
Nanocapsules / chemistry*
Phosphatidylcholines / chemical synthesis*
Chemical
Reg. No./Substance:
0/Liposomes; 0/Nanocapsules; 0/Phosphatidylcholines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of magnesium content in the feed on cataract development in Shumiya cataract rat.
Next Document:  Inhibitory effect of sulfoquinovosyl diacylglycerol on prokaryotic DNA polymerase I activity and cel...