| Efficient polycyclic aromatic hydrocarbons dihydroxylation in direct micellar systems. | |
| | |
MedLine Citation:
|
PMID: 11400097 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Optimization of whole-cell bioconversion of the polycyclic aromatic hydrocarbons (PAHs) anthracene, phenanthrene, and naphthalene to the enantiomerically pure corresponding cis-dihydroxydihydro derivatives by the Escherichia coli JM109 (pPS1778) recombinant strain, carrying the naphthalene dioxygenase and corresponding regulatory genes cloned from Pseudomonas fluorescens N3, in micellar systems, is presented. We show that direct microemulsion systems, where a nonionic surfactant such as 1.5% (v/v) Triton X-100 plus 0.6% to 1.0% (v/v) selected oils are able to solubilize the PAHs tested at relatively high concentrations (initial concentrations in the reaction medium > or =10 mM for naphthalene and phenanthrene and > or =2 mM for anthracene), and allow for more efficient substrate bioconversion. These media, while not affecting bacteria viability and performance, provide increased efficiency and final product yields (100% for naphthalene, >30% for anthracene, >60% for phenanthrene). The phase behavior of the direct microemulsion systems for the different substrates and oils utilized was monitored as a function of their volume fraction by light scattering experiments, and related to the bioconversion results. For anthracene and phenanthrene, the dihydroxylated products have an inhibitory effect on the conversion reactions, thus hindering complete turnover of the substrates. We ascertain that such inhibition is reversible because removal of the products formed allowed the process to start over at rates comparable to initial rates. To allow for complete conversion of the PAHs tested a stepwise or continuous separation of the product formed from the micellar reaction environment is being developed. |
| | |
Authors:
|
D Randazzo; D Berti; F Briganti; P Baglioni; A Scozzafava; P Di Gennaro; E Galli; G Bestetti |
Related Documents
:
|
19026617 - 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase as a tool for isolation and char... 20525857 - Isolation and identification of persistent chlorinated organophosphorus flame retardant... 7986057 - Identification of a heroin esterase in rhodococcus sp. strain h1. 15691947 - Unique kinetic properties of phenol-degrading variovorax strains responsible for effici... 7514147 - O-serogrouping and surface components of aeromonas hydrophila and aeromonas jandaei pat... 7137107 - Performance of yeast identification systems. an analysis of the college of american pat... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biotechnology and bioengineering Volume: 74 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2001 Aug |
Date Detail:
|
Created Date: 2001-06-11 Completed Date: 2001-10-04 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
|
Languages: eng Pagination: 240-8 Citation Subset: IM |
Copyright Information:
|
Copyright 2001 John Wiley & Sons, Inc. |
Affiliation:
|
Laboratorio di Chimica Bioinorganica, Dipartimento di Chimica, Università di Firenze, Via Della Lastruccia 5, 50019 Florence, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anthracenes
/
chemistry,
metabolism Escherichia coli / genetics, metabolism* Industrial Microbiology / methods* Micelles* Octoxynol / chemistry Oils / chemistry Phenanthrenes / chemistry, metabolism Polycyclic Hydrocarbons, Aromatic / chemistry*, metabolism* Surface-Active Agents / chemistry |
| Chemical | |
Reg. No./Substance:
|
0/Anthracenes; 0/Micelles; 0/Oils; 0/Phenanthrenes; 0/Polycyclic Hydrocarbons, Aromatic; 0/Surface-Active Agents; 120-12-7/anthracene; 85-01-8/phenanthrene; 9002-93-1/Octoxynol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Improved glycosylation of a foreign protein by Tn-5B1-4 cells engineered to express mammalian glycos...
Next Document: Enzyme stabilization by covalent binding in nanoporous sol-gel glass for nonaqueous biocatalysis.