Document Detail

Efficient photodynamic action of Victoria blue BO against the human leukemic cell lines K-562 and TF-1.
MedLine Citation:
PMID:  8234469     Owner:  NLM     Status:  MEDLINE    
Photodynamic induced cytotoxicity by Victoria blue BO (VB-BO), merocyanine 540 (MC540), Nile blue A (NB) and 4-tetrasulfonatophenyl-porphyrin (4-TSPP) has been studied on two human leukemic cell lines: K-562 and TF-1. Cells were incubated with dyes and irradiated with different doses of white light. Cell survival was assessed by propidium iodide (PI) staining using flow cytometry analysis. Concentrations of 5 x 10(-8) M VB-BO were found to kill 75% of cells, and a concentration of 1 x 10(-7) M induced more than 99% of cell killing. To obtain the same cytotoxic level, the presence of 2.6 x 10(-5) M of MC540 during irradiation was needed. Under the conditions used, NB was ineffective as a photosensitizer, although uptake studies showed that this dye was taken by the cells in much greater amounts than any other studied dye. Cell cycle distribution of TF-1 cells, surviving MC540 or VB-BO photosensitization has been studied by flow cytometry analysis after staining with Hoechst 33342 and PI. It was found that cells in G1 phase were slightly more resistant toward MC540- and VB-BO-mediated photosensitization than cells in other phases of the cell cycle.
M Fiedorowicz; J R Galindo; M Julliard; P Mannoni; M Chanon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  58     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-12-14     Completed Date:  1993-12-14     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  356-61     Citation Subset:  IM    
Laboratoire d'Activation, Mécanime, Modélisation Moléculaire: AMP-URA CNRS 1411, Faculté des Sciences Saint Jérôme, Marseille, France.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Cell Cycle / drug effects
Drug Screening Assays, Antitumor
Oxazines / pharmacology
Photosensitizing Agents / pharmacology
Porphyrins / pharmacology
Pyrimidinones / pharmacology
Quaternary Ammonium Compounds / pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents; 0/Oxazines; 0/Photosensitizing Agents; 0/Porphyrins; 0/Pyrimidinones; 0/Quaternary Ammonium Compounds; 2381-85-3/Nile Blue; 2390-60-5/Victoria blue BO; 35218-75-8/tetraphenylporphine sulfonate; 58823-12-4/merocyanine dye

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