Document Detail


Efficient multi-site-directed mutagenesis directly from genomic template.
MedLine Citation:
PMID:  23151786     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In this article, the traditional multi-site-directed mutagenesis method based on overlap extension PCR was improved specifically for complicated templates, such as genomic sequence or complementary DNA. This method was effectively applied for multi-site-directed mutagenesis directly from mouse genomic DNA, as well as for combination, deletion or insertion of DNA fragments.
Authors:
Fengtao Luo; Xiaolan Du; Tujun Weng; Xuan Wen; Junlan Huang; Lin Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biosciences     Volume:  37     ISSN:  0973-7138     ISO Abbreviation:  J. Biosci.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100809     Medline TA:  J Biosci     Country:  India    
Other Details:
Languages:  eng     Pagination:  965-9     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Trauma, Burns and Combined Injury, Center of Bone Metabolism and Repair, Trauma Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042 China.
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