Document Detail

Efficient modification of intracytoplasmic sperm injection technique for cases with total lack of sperm movement.
MedLine Citation:
PMID:  9222006     Owner:  NLM     Status:  MEDLINE    
A rapid, simple and efficient method for selecting living spermatozoa for intracytoplasmic sperm injection (ICSI) in cases with total lack of sperm movement is described. The selection is based on a characteristic deformation of living spermatozoa exposed to hypo-osmotic conditions during short sequential exposures to modified culture medium and polyvinylpyrrolidone solution; the osmolarity of both of these solutions is reduced by one half by diluting them with an equal amount of water. The application of the sperm viability selection step in six ICSI treatment cycles with total absence of sperm movement resulted in a fertilization rate of 41.9% and the establishment of two ongoing clinical pregnancies. The method described for the selection of living spermatozoa makes it possible to reach acceptable fertilization rates and to obtain ongoing pregnancies by ICSI in cases with total lack of sperm movement. Because of its simplicity, this method can easily be improvised when the total lack of sperm movement is an unexpected finding made on the day of the planned ICSI.
A Barros; M Sousa; T Angelopoulos; J Tesarik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  12     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-09-24     Completed Date:  1997-09-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1227-9     Citation Subset:  IM    
Laboratory of Medical Genetics, Institute of Biomedical Sciences, Faculty of Medicine, University of Porto, Portugal.
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MeSH Terms
Cell Separation
Cell Survival
Fertilization in Vitro / methods*
Oligospermia / pathology,  physiopathology,  therapy*
Osmotic Pressure
Sperm Motility
Spermatozoa* / pathology,  physiology

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