| Efficient media for high menaquinone-7 production: response surface methodology approach. | |
| | |
MedLine Citation:
|
PMID: 21839866 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The aim of this study was to assess the effect of nutrients on the production of menaquinone-7 and determine the optimum conditions to achieve a high concentration of this product. Bacillus subtilis natto was fermented at 40°C for a period of six days. Design of experiments was used for screening the most effective nutrients, and central composite face design was employed for the optimization. The optimum media consisted of 5% (w/v) yeast extract; 18.9% (w/v) soy peptone; 5% (w/v) glycerol and 0.06% (w/v) K(2)HPO(4). The pH, bacterial growth, concentrations of amino acids, glycerol and menaquinone-7 were measured at the optimum fermentation media each day. Total free amino acids concentration increased 1.7-fold during the fermentation. Lysine and glutamic acid were the most abundant whereas arginine, asparagine and serine were the limiting amino acids at the end of fermentation period. The menaquinone-7 concentration approached 86% of the final value in the third day of fermentation, where the bacteria growth was at exponential phase. At this condition the concentration of glycerol as carbon source and asparagines, serine and arginine as the amino acid source were dramatically diminished in the fermentation media. The optimum menaquinone-7 concentration was in good agreement with the predicted value by the model (96% validity). Maximum menaquinone-7 concentration of 62.32±0.34mg/L was achieved after six days of fermentation; this value is the highest concentration reported in the literature. |
| | |
Authors:
|
Aydin Berenjian; Raja Mahanama; Andrea Talbot; Ray Biffin; Hubert Regtop; Peter Valtchev; John Kavanagh; Fariba Dehghani |
Related Documents
:
|
848926 - Prevention of 5-fluorouracil-caused growth inhibition in sordaria fimicola. 8469176 - Antifungal activity of myxococcus species 1 production, physicochemical and biological ... 7820306 - Influence of fatty acids and bovine serum albumin on the growth of human hepatoma and i... 16659316 - Studies on the growth effects of the canaline-urea cycle amino acids with lemna minor l. 7815276 - Effect of topical preparation of mycophenolic acid on experimental allergic contact der... 8886606 - Comparative metabolism of the nonsteroidal antiinflammatory drug, aceclofenac, in the r... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-8-3 |
Journal Detail:
|
Title: New biotechnology Volume: - ISSN: 1876-4347 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
|
Created Date: 2011-8-15 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101465345 Medline TA: N Biotechnol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier B.V. |
Affiliation:
|
School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW 2006, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Fibrin matrix-supported three-dimensional organ culture of adipose tissue for selective outgrowth, e...
Next Document: Phosphoproteomics: Searching for a needle in a haystack.