| Efficient lentiviral transduction of liver requires cell cycling in vivo. | |
| | |
MedLine Citation:
|
PMID: 10615126 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human-immunodeficiency-virus (HIV)-based lentiviral vectors are a promising tool for in vivo gene therapy. Unlike Moloney-murine-leukaemia-based retroviruses (MLV), lentiviruses are believed to stably transduce quiescent (non-cycling) cells in various organs. No previous studies, however, have directly established the cell-cycle status of any transduced cell type at the time of vector administration in vivo. In vitro studies using wild-type HIV or HIV-based vectors have shown that, in some cases, cell-cycle activation is required for infection, even though cellular mitosis is not an absolute requirement for integration. Even if the block in reverse transcription is overcome in quiescent T cells, productive infection by HIV cannot be rescued in the absence of cell-cycle activation. The potential use of these vectors for gene therapy prompted our study, which establishes a cell-cycle requirement for efficient transduction of hepatocytes in vivo. |
| | |
Authors:
|
F Park; K Ohashi; W Chiu; L Naldini; M A Kay |
Related Documents
:
|
10629116 - Cancer and the cell cycle. 12498076 - Cell cycle and its parameters in flowering plants. 16661676 - Control of pyrimidine biosynthesis in synchronously dividing cells of helianthus tubero... 11124426 - Familial alzheimer's disease presenilin-1 mutants potentiate cell cycle arrest. 20452346 - Acetylation of core histones in response to hdac inhibitors is diminished in mitotic he... 20861156 - Nanosilver particle effects on drug metabolism in vitro. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Nature genetics Volume: 24 ISSN: 1061-4036 ISO Abbreviation: Nat. Genet. Publication Date: 2000 Jan |
Date Detail:
|
Created Date: 2000-02-10 Completed Date: 2000-02-10 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9216904 Medline TA: Nat Genet Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 49-52 Citation Subset: IM; X |
Affiliation:
|
Department of Pediatrics, Stanford University, Stanford, California, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Cycle / genetics* Cell Line Female Genetic Vectors Humans Lentivirus / genetics* Liver / cytology, enzymology, metabolism* Mice Mice, Inbred C57BL Mice, SCID Rats Rats, Inbred F344 Transduction, Genetic* |
| Grant Support | |
ID/Acronym/Agency:
|
R01-HL53682/HL/NHLBI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mutations in TNFRSF11A, affecting the signal peptide of RANK, cause familial expansile osteolysis.
Next Document: Novel dominant mutations in Saccharomyces cerevisiae MSH6.