| Efficient introduction of phosphorothioates into RNA oligonucleotides by 3-ethoxy-1,2,4-dithiazoline-5-one (EDITH). | |
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MedLine Citation:
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PMID: 8836195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We recently reported on the use of 1,2,4-dithiazolidine-3,5-dione (DtsNH) and 3-ethoxy-1,2,4-dithiazoline-5-one (EDITH) as effective sulfurizing reagents for the preparation of phosphorothioate-containing oligodeoxyribo-nucleotides [Xu et al. (1996) Nucleic Acids Res., 24, 1602-1607]. One challenge in automated solid-phase synthesis of phosphorothioate-containing RNA is to develop sulfurization reagents that are effective in the presence of bulky 2'-OH protecting groups. The present study demonstrates that EDITH is exceedingly effective at low concentrations (0.05 M) and short reaction times (2 min) for the automated synthesis of oligoribonucleotides. |
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Authors:
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Q Xu; G Barany; R P Hammer; K Musier-Forsyth |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nucleic acids research Volume: 24 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1996 Sep |
Date Detail:
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Created Date: 1996-11-07 Completed Date: 1996-11-07 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3643-4 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Minnesota, Minneapolis 55455-0431, USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Chromatography, High Pressure Liquid Molecular Sequence Data RNA / chemical synthesis* Thiazoles* Thionucleotides / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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GM 28934/GM/NIGMS NIH HHS; GM 42722/GM/NIGMS NIH HHS; GM 43552/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/3-ethoxy-1,2,4-dithiazoline-5-one; 0/Thiazoles; 0/Thionucleotides; 63231-63-0/RNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 15 Month: 9 Year: 1996 Volume: 24 Issue: 18 First Page: 3643 Last Page: 3644 ID: 146137 PubMed Id: 8836195 Publisher Item Identifier: l60193 |
| Efficient introduction of phosphorothioates into RNA oligonucleotides by 3-ethoxy-1,2,4-dithiazoline-5-one (EDITH). | |
| Q Xu | |
| G Barany | |
| R P Hammer | |
| K Musier-Forsyth | |
| Department of Chemistry, University of Minnesota, Minneapolis 55455-0431, USA. |
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