| Efficient intracytoplasmic labeling of human umbilical cord blood mesenchymal stromal cells with ferumoxides. | |
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MedLine Citation:
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PMID: 18088004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mesenchymal stromal cells (MSCs) are multipotent cells found in several adult tissues; they have the capacity to differentiate into mesodermal, ectodermal, and endodermal tissues in vitro. There have been several reports that MSCs have therapeutic effects in a variety of diseases. Therefore, using a cell labeling technique, monitoring their temporal and spatial migration in vivo, would be useful in the clinical setting. Magnetic resonance imaging (MRI)--tracking of superparamagnetic iron oxide (SPIO)-labeled cells--is a noninvasive technique for determining the location and migration of transplanted cells. In the present study, we evaluated the influence and toxicity of SPIO (ferumoxides) labeling on multiple differentiated MSCs. To evaluate the influence and toxicity of ferumoxides labeling on differentiation of MSCs, a variety of concentrations of ferumoxides were used for labeling MSCs. We found that the cytoplasm of adherent cells was effectively labeled at low concentrations of ferumoxides. Compared with unlabeled controls, the ferumoxides-labeled MSCs exhibited a similar proliferation rate and apoptotic progression. The labeled MSCs differentiated into osteoblasts and adipocytes in an identical fashion as the unlabeled cells. However, chondrogenesis and neurogenesis were inhibited at high concentrations of ferumoxides. Our results suggest the effective concentration for ferumoxides use in tracking MSCs. |
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Authors:
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Jae Kwon Lee; Man Kyoung Lee; Hye Jin Jin; Dal-Soo Kim; Yoon Sun Yang; Wonil Oh; Sung-Eun Yang; Tae Seok Park; Soo Yeol Lee; Bum-Soo Kim; Sin-Soo Jeun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell transplantation Volume: 16 ISSN: 0963-6897 ISO Abbreviation: Cell Transplant Publication Date: 2007 |
Date Detail:
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Created Date: 2007-12-19 Completed Date: 2008-04-14 Revised Date: 2013-04-11 |
Medline Journal Info:
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Nlm Unique ID: 9208854 Medline TA: Cell Transplant Country: United States |
Other Details:
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Languages: eng Pagination: 849-57 Citation Subset: IM |
Affiliation:
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Medipost Biomedical Research Institute, Medipost Co. Ltd., Asan Institute of Life Science, Seoul, 138-736, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Nuclear
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metabolism Apoptosis / drug effects Blotting, Western Cell Differentiation / drug effects Cell Proliferation / drug effects Collagen Type II / genetics Dextrans Dose-Response Relationship, Drug Ferumoxytol / pharmacology Fetal Blood / cytology* Flow Cytometry Gene Expression / drug effects Humans Iron / pharmacology* Magnetic Resonance Imaging Magnetite Nanoparticles Nerve Tissue Proteins / metabolism Oxides / pharmacology* Reverse Transcriptase Polymerase Chain Reaction Stromal Cells / cytology, drug effects* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Nuclear; 0/Collagen Type II; 0/Magnetite Nanoparticles; 0/Nerve Tissue Proteins; 0/Oxides; 0/neuronal nuclear antigen NeuN, human; 1317-61-9/Ferumoxytol; 7439-89-6/Iron; 9004-54-0/Dextrans; G6N3J05W84/ferumoxides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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