| Efficient growth suppression and apoptosis in human laryngeal carcinoma cell line HEP-2 induced by an adeno-associated virus expressing human FAS ligand. | |
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MedLine Citation:
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PMID: 22267220 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: Apoptosis induced by Fas/FasL system has been proposed as a gene therapy methold for various cancers. METHODS: We used adeno-associated virus-expressing enhanced green fluorescent protein (EGFP)-human FasL (AAV-EGFP-hFasL) to deliver FasL into Hep-2 cells, cytotoxicity was detected by MTS assay , apoptosis was confirmed by flow cytometry. We also treated the xenograft of Hep-2 tumor in nude mice with intratumoral injection of AAV-EGFP-hFasL. The size of the xenograft, the apoptosis in the xenograft, and the survival rate of the inoculated mice were then evaluated. RESULTS: Hep-2 cells infected with AAV-EGFP-hFasL showed increased apoptosis rate and killing effect compared with AAV-EGFP-infected cells. In addition intratumoral injections of AAV-EGFP-hFasL into Hep-2 xenografts induced significant growth suppression of tumors. CONCLUSION: Our findings suggest that the introduction of FasL into head and neck squamous cell carcinoma may induce significant apoptosis, and adeno-associated virus may be a useful vehicle for gene therapy. © 2012 Wiley Periodicals, Inc. Head Neck, 2012. |
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Authors:
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Haili Sun; Yuhe Liu; Dingfang Bu; Xiang Liu; James S Norris; Shuifang Xiao |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-20 |
Journal Detail:
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Title: Head & neck Volume: - ISSN: 1097-0347 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8902541 Medline TA: Head Neck Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Department of Otolaryngology-Head & Neck Surgery, Peking University First Hospital, Beijing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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