Document Detail


Efficient differentiation of human embryonic stem cells into functional cerebellar-like cells.
MedLine Citation:
PMID:  20521974     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The cerebellum has critical roles in motor and sensory learning and motor coordination. Many cerebellum-related disorders indicate cell therapy as a possible treatment of neural loss. Here we show that application of inductive signals involved in early patterning of the cerebellar region followed by application of different factors directs human embryonic stem cell differentiation into cerebellar-like cells such as granule neurons, Purkinje cells, interneuron, and glial cells. Neurons derived using our protocol showed a T-shaped polarity phenotype and express similar markers to the developed human cerebellum. Electrophysiological measurements confirmed functional electrical properties compatible with these cells. In vivo implantation of differentiated human embryonic stem cells transfected with MATH1-GFP construct into neonatal mice resulted in cell migration across the molecular and the Purkinje cell layers and settlement in the internal molecular layers. Our findings demonstrate that the universal mechanisms involved in the development of cerebellum can be efficiently recapitulated in vitro, which enables the design of new strategies for cell replacement therapy, to study early human development and pathogenesis of neurodegenerative diseases.
Authors:
Slaven Erceg; Mohammad Ronaghi; Ivan Zipancic; Sergio Lainez; Mireia Gárcia Roselló; Chen Xiong; Victoria Moreno-Manzano; Fernando Javier Rodríguez-Jiménez; Rosa Planells; Manuel Alvarez-Dolado; Shom Shanker Bhattacharya; Miodrag Stojkovic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-07
Journal Detail:
Title:  Stem cells and development     Volume:  19     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1745-56     Citation Subset:  IM    
Affiliation:
Cellular Reprogramming Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain. serceg@cipf.es
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