Document Detail


Efficient differentiation of human embryonic stem cells to definitive endoderm.
MedLine Citation:
PMID:  16258519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The potential of human embryonic stem (hES) cells to differentiate into cell types of a variety of organs has generated much excitement over the possible use of hES cells in therapeutic applications. Of great interest are organs derived from definitive endoderm, such as the pancreas. We have focused on directing hES cells to the definitive endoderm lineage as this step is a prerequisite for efficient differentiation to mature endoderm derivatives. Differentiation of hES cells in the presence of activin A and low serum produced cultures consisting of up to 80% definitive endoderm cells. This population was further enriched to near homogeneity using the cell-surface receptor CXCR4. The process of definitive endoderm formation in differentiating hES cell cultures includes an apparent epithelial-to-mesenchymal transition and a dynamic gene expression profile that are reminiscent of vertebrate gastrulation. These findings may facilitate the use of hES cells for therapeutic purposes and as in vitro models of development.
Authors:
Kevin A D'Amour; Alan D Agulnick; Susan Eliazer; Olivia G Kelly; Evert Kroon; Emmanuel E Baetge
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Publication Detail:
Type:  Journal Article     Date:  2005-10-28
Journal Detail:
Title:  Nature biotechnology     Volume:  23     ISSN:  1087-0156     ISO Abbreviation:  Nat. Biotechnol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-07     Completed Date:  2006-02-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9604648     Medline TA:  Nat Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1534-41     Citation Subset:  IM    
Affiliation:
CyThera Inc., 3550 General Atomics Ct., San Diego, California 92121, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods*
Cell Differentiation
Cell Proliferation
Cells, Cultured
Endoderm / cytology*,  physiology*
Humans
Mice
Stem Cells / cytology*,  physiology*
Tissue Engineering / methods*
Comments/Corrections
Comment In:
Nat Biotechnol. 2005 Dec;23(12):1491-2   [PMID:  16333288 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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