Document Detail


Efficient automated assessment of genetic abnormalities detected by fluorescence in situ hybridization on brush cytology in a Barrett esophagus surveillance population.
MedLine Citation:
PMID:  17385213     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Automated assessment of genetic abnormalities detected by fluorescence in situ hybridization (FISH) in brush cytology specimens from patients with Barrett esophagus (BE) may enhance the clinical applicability of this methodology. The objectives of this study were to validate a novel, automated, proprietary system (CytoVison SPOT AX) for the assessment of FISH abnormalities in BE brush cytology and, subsequently, to use this automated method for screening of a BE surveillance cohort. METHODS: FISH with DNA probes for chromosomes 9, 17, and Y, and for the 9p21 (p16), 17q11.2 (Her2/neu), and 17p13.1 (p53) loci was applied on brush cytology specimens from a surveillance cohort of 151 patients with BE. Validation of the automated system was performed by comparison of the automated FISH results with manual scores for the first 60 patients. RESULTS: There was 98% concordance between manual and automated FISH analysis with kappa values from 0.49 to 1 for the different probes. The loss of 17p13.1 (p53) was observed in only 5% of patients with no dysplasia (ND) and in 9% of patients with low-grade dysplasia (LGD) but increased to 46% in patients with high-grade dysplasia (HGD) (P < .005; Fisher exact test). Chromosomes 9 and 17 were observed in 6% of patients with ND, in 21% of patients with LGD, and in 62% of patients with HGD (P < .05). Ten percent of patients with ND had loss of the Y chromosome, which increased to 27% in patients with HGD (P< .05). The amplification of 17q11.2 (Her2/neu) was detected in 62% of patients with HGD (P < .001). CONCLUSIONS: The current investigation indicated that the CytoVison SPOT AX is an objective, efficient system for the analysis of DNA-FISH on BE brush cytology and is applicable for analyzing large populations of BE patients. In the current study cohort, the loss of 17p13.1 (p53), Y chromosome loss, and polysomy of chromosomes 17 and 9 were correlated with increasing grade of dysplasia in patients with BE.
Authors:
Agnieszka M Rygiel; Jantine W P M van Baal; Francesca Milano; Kenneth K Wang; Febo J ten Kate; Paul Fockens; Wilda D Rosmolen; Jacques J G H M Bergman; Maikel P Peppelenbosch; Kausilia K Krishnadath
Related Documents :
22725983 - Borden-shucart type i dural arteriovenous fistulas: clinical course including risk of c...
21604063 - Renal function assessment in patients with systemic lupus erythematosus.
6797343 - Hyperprolactinaemia in polycystic ovary syndrome.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Cancer     Volume:  109     ISSN:  0008-543X     ISO Abbreviation:  Cancer     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-03     Completed Date:  2007-06-07     Revised Date:  2007-07-12    
Medline Journal Info:
Nlm Unique ID:  0374236     Medline TA:  Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1980-8     Citation Subset:  AIM; IM    
Copyright Information:
(c) 2007 American Cancer Society
Affiliation:
Department of Experimental Internal Medicine, Academic Medical Center, Amsterdam, the Netherlands. a.lukuc@amc.uva.nl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Automation
Barrett Esophagus / genetics*,  pathology
Chromosome Aberrations*
Chromosomes, Human, Pair 17 / genetics
Chromosomes, Human, Pair 9 / genetics
Chromosomes, Human, Y / genetics
Cytogenetic Analysis*
Cytological Techniques
Female
Genes, erbB-2 / genetics
Genes, p16
Genes, p53 / genetics
Humans
In Situ Hybridization, Fluorescence / instrumentation,  methods*
Male
Middle Aged

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Role of caspase-8 in hepatocyte response to infection and injury in mice.
Next Document:  The burden of liver cancer in Asians and Pacific Islanders in the Greater San Francisco Bay Area, 19...