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Efficient activation of transcription in yeast by the BPV1 E2 protein.
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MedLine Citation:
PMID:  2539584     Owner:  NLM     Status:  MEDLINE    
The full-length gene product encoded by the E2 open reading frame (ORF) of bovine papillomavirus type 1 (BPV1) is a transcriptional transactivator. It is believed to mediate its effect on the BPV1 long control region (LCR) by binding to motifs with the consensus sequence ACCN6GGT. The minimal functional cis active site, called the E2 response element (E2RE), in mammalian cells comprises two copies of this motif. Here we have shown that E2 can function in Saccharomyces cerevisiae by placing an E2RE upstream of a synthetic yeast assay promoter which consists of a TATA motif and an mRNA initiation site, spaced correctly. This E2RE-minimal promoter is only transcriptionally active in the presence of E2 protein and the resulting mRNA is initiated at the authentic start site. This is the first report of a mammalian viral transactivator functioning in yeast. The level of activation by E2 via the E2RE was the same as observed with the highly efficient authentic PGK promoter where the upstream activation sequence is composed of three distinct elements. Furthermore a single E2 motif which is insufficient in mammalian cells as an activation site was as efficiently utilized in yeast as the E2RE (2 motifs). Previous studies have shown that mammalian cellular activators can function in yeast and our data now extend this to viral-specific activators. Our data indicate however that while the mechanism of transactivation is broadly conserved there may be significant differences at the detailed level.
C A Stanway; M P Sowden; L E Wilson; A J Kingsman; S M Kingsman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  17     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-05-22     Completed Date:  1989-05-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2187-96     Citation Subset:  IM    
Department of Biochemistry, Oxford University, UK.
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MeSH Terms
Base Sequence
Bovine papillomavirus 1
DNA, Fungal / metabolism
DNA-Binding Proteins / metabolism,  pharmacology*
Enhancer Elements, Genetic
Interferon Type I / genetics
Molecular Sequence Data
Promoter Regions, Genetic
RNA, Messenger / genetics
Saccharomyces cerevisiae / genetics*
Transcription, Genetic / drug effects*
Viral Proteins / metabolism,  pharmacology*
Reg. No./Substance:
0/DNA, Fungal; 0/DNA-Binding Proteins; 0/E2 protein, Bovine papillomavirus; 0/Interferon Type I; 0/RNA, Messenger; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 25 Month: 3 Year: 1989
Volume: 17 Issue: 6
First Page: 2187 Last Page: 2196
ID: 317588
PubMed Id: 2539584

Efficient activation of transcription in yeast by the BPV1 E2 protein.
C A Stanway
M P Sowden
L E Wilson
A J Kingsman
S M Kingsman
Department of Biochemistry, Oxford University, UK.

Article Categories:
  • Research Article

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