Document Detail


Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment.
MedLine Citation:
PMID:  18295342     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Schwann cell purification is usually difficult due to the contamination of fibroblasts, which often become a predominant cell type in Schwann cell culture in vitro. We have developed a novel and efficient method to enrich Schwann cells by differential detachment of two types of cells. In the culture, cells were treated with a multiplex collagenase and the Schwann cells were found to detach faster than fibroblasts and thus Schwann cells could be easily isolated. Within 5 days, Schwann cell purity could reach above 99%, which was confirmed by immunostaining characterization and flow cytometric analysis. In addition, this efficient method can reach a high cell yield after two rounds of differential detachment procedures and does not require antimitotic treatment or special equipment as often needed by other reported methods.
Authors:
Yu-Qing Jin; Wei Liu; Tan-Hui Hong; Yilin Cao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-16
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  170     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-03-31     Completed Date:  2008-07-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  140-8     Citation Subset:  IM    
Affiliation:
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Tissue Engineering Laboratory, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count
Cell Separation / methods*
Cells, Cultured
Collagenases / pharmacology*
Fibroblasts / drug effects,  physiology
Flow Cytometry
Fluorescent Antibody Technique
Immunohistochemistry
Mice
Mice, Inbred C57BL
Receptor, Nerve Growth Factor / physiology
Schwann Cells / drug effects,  physiology*
Chemical
Reg. No./Substance:
0/Receptor, Nerve Growth Factor; EC 3.4.24.-/Collagenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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