Document Detail


Efficient RecABC-dependent, homologous recombination between coliphage lambda and plasmids requires a phage ninR region gene.
MedLine Citation:
PMID:  2963943     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A phage lambda gene that gives a 100-fold increase in recombinant frequencies for RecABC pathway-mediated, phage-plasmid homologous recombination (Shen and Huang 1986) maps to ninG (orf 204) of lambda. We call this gene rap, for recombination adept with plasmid. A similar determinant exists in Charon 4A and maps in phi 80-derived sequences, between nin5 and the Rz homology with lambda. The absence of the Rap+ phenotype from certain lambda vectors explains the inefficiency of screening the resulting phage libraries using phage-plasmid homologous recombination. The mapping of rap permits the construction of lambda vectors more suitable for this screening technique.
Authors:
W C Hollifield; E N Kaplan; H V Huang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  210     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-03-30     Completed Date:  1988-03-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  248-55     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, MO 63110.
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MeSH Terms
Descriptor/Qualifier:
Bacteriophage lambda / genetics*
Chromosome Mapping
Escherichia coli / genetics
Exodeoxyribonuclease V
Exodeoxyribonucleases / genetics
Genes, Viral*
Plasmids*
Rec A Recombinases / genetics
Recombination, Genetic*
Grant Support
ID/Acronym/Agency:
GM31838/GM/NIGMS NIH HHS; T32GM07200/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
EC 2.7.7.-/Rec A Recombinases; EC 3.1.-/Exodeoxyribonucleases; EC 3.1.11.5/Exodeoxyribonuclease V

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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