Document Detail


Efficient RGD-independent entry process of coxsackievirus A9.
MedLine Citation:
PMID:  8920824     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previously we showed for coxsackievirus A9 (CAV-9) that specific interactions between the RGD motif of capsid protein VP1 and the alpha v beta 3 integrin are involved in virus binding and entry into green monkey kidney cells (GMK) and some other cell lines. The RGD-recognizing alpha v beta 3 integrin is known as the vitronectin receptor (VNR). During replication in the gut, CAV-9 like all other enteroviruses are exposed to host proteolytic enzymes, and we showed previously that the RGD-containing 15 amino acids long carboxy terminal extension of VP1 is cleaved off by trypsin. The trypsin-treated CAV-9 was still infectious, although at an apparently reduced level as assessed in GMK cells. This indicated that the virus was able to bypass the RGD-dependent entry and possibly use an alternative receptor. We have now found that in RD cells, a human rhabdomyosarcoma cell line, neither RGD-containing oligopeptides nor polyclonal antiserum to VNR are able to protect the cells from CAV-9 infection suggesting that the RGD motif is not involved in binding or entry of the virus into these cells. This result was further confirmed by demonstrating that, in RD cells, the trypsin-treated CAV-9 lacking the RGD-containing insert appeared to be as infectious as the untreated virus. The most striking difference between the virus receptors in the RD and the GMK cells was seen when the rate of virus uncoating was studied. For virus particles bound to the RD cells, the uncoating step started already at 18-20 degrees C and the process went on rapidly at 36 degrees C resulting in complete disintegration of cell-bound virions. In contrast, alpha v beta 3-bound virus particles in the GMK cells appeared to uncoat slowly even at 36 degrees C and during the 90 min observation period only a small, hardly visible fraction was found to be disintegrated. Trypsin-cleaved CAV-9 showed the rapid disintegration kinetics in GMK cells as well suggesting that these cells contain both types of receptor specificity. These results indicate that CAV-9 is able to use two different entry routes into host cells depending on the target cells and on phenotypic properties of the virus regulated by host proteases.
Authors:
M Roivainen; L Piirainen; T Hovi
Related Documents :
2535724 - Isolation and characterization of mouse fm3a cell mutants which are devoid of newcastle...
179634 - The production of foot-and-mouth disease virus from bhk 21 c 13 cells grown on the surf...
2844844 - Isolation and propagation of enteric adenoviruses in hep-2 cells.
14581524 - Hep3b human hepatoma cells support replication of the wild-type and a 5'-end deletion m...
8652654 - Activation of acyl-coenzyme a:cholesterol acyltransferase activity by cholesterol is no...
19340424 - Breast tumor cells isolated from in vitro resistance to trastuzumab remain sensitive to...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of virology     Volume:  141     ISSN:  0304-8608     ISO Abbreviation:  Arch. Virol.     Publication Date:  1996  
Date Detail:
Created Date:  1996-12-26     Completed Date:  1996-12-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  AUSTRIA    
Other Details:
Languages:  eng     Pagination:  1909-19     Citation Subset:  IM    
Affiliation:
Enterovirus Laboratory, National Public Health Institute, Helsinki, Finland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Capsid / metabolism*
Capsid Proteins
Cell Line
Cercopithecus aethiops
Enterovirus / metabolism*,  pathogenicity
Humans
Oligopeptides / metabolism
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/Oligopeptides; 99896-85-2/arginyl-glycyl-aspartic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of adenovirus hexons by their epitope composition.
Next Document:  Selection of hepatitis C virus quasispecies during interferon treatment.