Document Detail

Efficient Production of d-Tagatose Using a Food-Grade Surface Display System.
MedLine Citation:
PMID:  24979201     Owner:  NLM     Status:  Publisher    
d-Tagatose, a functional sweetener, is commonly transformed from d-galactose by l-arabinose isomerase (l-AI). In this study, a novel type of biocatalyst, l-AI from Lactobacillus fermentum CGMCC2921 displayed on the spore surface of Bacillus subtilis 168, was developed for producing d-tagatose. The anchored l-AI, exhibiting the relatively high bioactivity, suggested that the surface display system using CotX as the anchoring protein was successfully constructed. The stability of the anchored l-AI was significantly improved. Specifically, the consolidation of thermal stability representing 87% of relative activity was retained even at 80 °C for 30 min, which remarkably favored the production of d-tagatose. Under the optimal conditions, the robust spores can convert 75% d-galactose (100 g/L) into d-tagatose after 24 h, and the conversion rate remained at 56% at the third cycle. Therefore, this biocatalysis system, which could express the target enzyme on the food-grade vector, was an alternative method for the value-added production of d-tagatose.
Yi Liu; Sha Li; Hong Xu; Lingtian Wu; Zheng Xu; Jing Liu; Xiaohai Feng
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-8
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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