| Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter. | |
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MedLine Citation:
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PMID: 19802745 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As the main structural protein of oil body, OLEOSIN is highly expressed only during seed development. OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulating seed development and seed-specific gene expression. Here, we first report how seed-specific B3 domain transcription factor leafy cotyledon2 (LEC2) efficiently activates OLEOSIN expression. The central promoter region of OLEOSIN, responsible for seed specificity and LEC2 activation, was determined by 5'-deletion analysis. Binding experiments in yeast cells and electrophoretic mobility shift assays showed that LEC2 specifically bound to two conserved RY elements in this region. In transient expression assays, mutation in either RY element dramatically reduced LEC2 activation of OLEOSIN promoter activity, while double mutation abolished it. Analysis of the distribution of RY elements in seed-specific genes activated by LEC2 also supported the idea that genes containing neighboring RY elements responded strongly to LEC2 activation. Therefore, we conclude that two neighboring RY elements are essential for efficient LEC2 activation of OLEOSIN expression. These findings will help us better utilize seed-specific promoter activity. |
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Authors:
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NanYing Che; Yang Yang; YanDong Li; LiLi Wang; Ping Huang; Yin Gao; ChengCai An |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-06 |
Journal Detail:
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Title: Science in China. Series C, Life sciences / Chinese Academy of Sciences Volume: 52 ISSN: 1006-9305 ISO Abbreviation: Sci. China, C, Life Sci. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-10-05 Completed Date: 2009-12-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9611809 Medline TA: Sci China C Life Sci Country: China |
Other Details:
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Languages: eng Pagination: 854-63 Citation Subset: IM |
Affiliation:
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The National Laboratory of Protein Engineering and Plant Genetic Engineering, School of Life Sciences, Peking University, Beijing 100871, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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genetics*,
metabolism* Arabidopsis Proteins / genetics*, metabolism* Base Sequence Binding Sites / genetics DNA Primers / genetics DNA, Plant / genetics, metabolism Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Plants, Genetically Modified Promoter Regions, Genetic Protein Binding Sequence Deletion Sequence Homology, Nucleic Acid Transcription Factors / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/DNA Primers; 0/DNA, Plant; 0/LEC2 protein, Arabidopsis; 0/Transcription Factors; 0/oleosin protein, Arabidopsis |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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