Document Detail


Efficient generation of virus-free iPS cells using liposomal magnetofection.
MedLine Citation:
PMID:  23049868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The generation of induced pluripotent stem (iPS) cells is a powerful tool in regenerative medicine, and advances in nanotechnology clearly have great potential to enhance stem cell research. Here, we introduce a liposomal magnetofection (LMF) method for iPS cell generation. Efficient conditions for generating virus-free iPS cells from mouse embryonic fibroblast (MEF) cells were determined through the use of different concentrations of CombiMag nanoparticle-DNA(pCX-OKS-2A and pCX-cMyc)-lipoplexes and either one or two cycles of the LMF procedure. The cells were prepared in a short reprogramming time period (≤ 8 days, 0.032-0.040%). Among the seven LMF-iPS cell lines examined, two were confirmed to be integration-free, and an integration-free LMF-iPS cell line was produced under the least toxic conditions (single LMF cycle with a half-dose of plasmid). This cell line also displayed in vitro/in vivo pluripotency, including teratoma formation and chimeric mouse production. In addition, the safety of CombiMag-DNA lipoplexes for the transfection of MEF cells was confirmed through lactate dehydrogenase activity assay and transmission electron microscopy. These results demonstrated that the LMF method is simple, effective, and safe. LMF may represent a superior technique for the generation of virus-free or integration-free iPS cell lines that could lead to enhanced stem cell therapy in the future.
Authors:
Hyo Young Park; Eun Hyung Noh; Hyung-Min Chung; Man-Jong Kang; Eun Young Kim; Se Pill Park
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-25
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-10     Completed Date:  2013-05-07     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e45812     Citation Subset:  IM    
Affiliation:
Miraebio Research Institute, Mirae Biotech, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques*
Cells, Cultured
DNA / chemistry,  metabolism
Genetic Techniques
Induced Pluripotent Stem Cells / cytology*
L-Lactate Dehydrogenase / metabolism
Liposomes / metabolism*
Magnetics
Mice
Microscopy, Electron, Transmission / methods
Models, Genetic
Plasmids / metabolism
Stem Cells / cytology
Teratoma / metabolism
Time Factors
Transgenes
Chemical
Reg. No./Substance:
0/Liposomes; 9007-49-2/DNA; EC 1.1.1.27/L-Lactate Dehydrogenase
Comments/Corrections

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