Document Detail


Efficient generation of nonhuman primate induced pluripotent stem cells.
MedLine Citation:
PMID:  21058905     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Induced pluripotent stem (iPS) cells have great potential for regenerative medicine and gene therapy. Thus far, iPS cells have typically been generated using integrating viral vectors expressing various reprogramming transcription factors; nonintegrating methods have been less effective and efficient. Because there is a significant risk of malignant transformation and cancer involved with the use of iPS cells, careful evaluation of transplanted iPS cells will be necessary in small and large animal studies before clinical application. Here, we have generated and characterized nonhuman primate iPS cells with the goal of evaluating iPS cell transplantation in a clinically relevant large animal model. We developed stable Phoenix-RD114-based packaging cell lines that produce OCT4, SOX2, c-MYC, and KLF4 (OSCK) expressing gammaretroviral vectors. Using these vectors in combination with small molecules, we were able to efficiently and reproducibly generate nonhuman primate iPS cells from pigtailed macaques (Macaca nemestrina). The established nonhuman primate iPS cells exhibited pluripotency and extensive self-renewal capacity. The facile and reproducible generation of nonhuman primate iPS cells using defined producer cells as a source of individual reprogramming factors should provide an important resource to optimize and evaluate iPS cell technology for studies involving stem cell biology and regenerative medicine.
Authors:
Bonan Zhong; Grant D Trobridge; Xiaobing Zhang; Korashon L Watts; Aravind Ramakrishnan; Martin Wohlfahrt; Jennifer E Adair; Hans-Peter Kiem
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Publication Detail:
Type:  Journal Article     Date:  2011-02-01
Journal Detail:
Title:  Stem cells and development     Volume:  20     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-06     Completed Date:  2011-09-13     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  795-807     Citation Subset:  IM    
Affiliation:
Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / genetics
Cell Line
Female
Fibroblasts / cytology,  metabolism*
Gammaretrovirus / genetics,  metabolism
Genetic Vectors / genetics,  metabolism
Humans
Induced Pluripotent Stem Cells* / cytology,  metabolism
Kruppel-Like Transcription Factors / genetics,  metabolism
Macaca nemestrina / genetics*,  metabolism
Male
Mice
Microarray Analysis
Nuclear Reprogramming* / genetics
Octamer Transcription Factor-3 / genetics,  metabolism
Proto-Oncogene Proteins c-myc / genetics,  metabolism
Regenerative Medicine
Reverse Transcriptase Polymerase Chain Reaction
Risk
SOXB1 Transcription Factors / genetics,  metabolism
Transfection
Chemical
Reg. No./Substance:
0/GKLF protein; 0/Kruppel-Like Transcription Factors; 0/Octamer Transcription Factor-3; 0/Proto-Oncogene Proteins c-myc; 0/SOX2 protein, human; 0/SOXB1 Transcription Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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