| Efficient generation of nonhuman primate induced pluripotent stem cells. | |
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MedLine Citation:
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PMID: 21058905 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Induced pluripotent stem (iPS) cells have great potential for regenerative medicine and gene therapy. Thus far, iPS cells have typically been generated using integrating viral vectors expressing various reprogramming transcription factors; nonintegrating methods have been less effective and efficient. Because there is a significant risk of malignant transformation and cancer involved with the use of iPS cells, careful evaluation of transplanted iPS cells will be necessary in small and large animal studies before clinical application. Here, we have generated and characterized nonhuman primate iPS cells with the goal of evaluating iPS cell transplantation in a clinically relevant large animal model. We developed stable Phoenix-RD114-based packaging cell lines that produce OCT4, SOX2, c-MYC, and KLF4 (OSCK) expressing gammaretroviral vectors. Using these vectors in combination with small molecules, we were able to efficiently and reproducibly generate nonhuman primate iPS cells from pigtailed macaques (Macaca nemestrina). The established nonhuman primate iPS cells exhibited pluripotency and extensive self-renewal capacity. The facile and reproducible generation of nonhuman primate iPS cells using defined producer cells as a source of individual reprogramming factors should provide an important resource to optimize and evaluate iPS cell technology for studies involving stem cell biology and regenerative medicine. |
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Authors:
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Bonan Zhong; Grant D Trobridge; Xiaobing Zhang; Korashon L Watts; Aravind Ramakrishnan; Martin Wohlfahrt; Jennifer E Adair; Hans-Peter Kiem |
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Publication Detail:
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Type: Journal Article Date: 2011-02-01 |
Journal Detail:
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Title: Stem cells and development Volume: 20 ISSN: 1557-8534 ISO Abbreviation: Stem Cells Dev. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-05-06 Completed Date: 2011-09-13 Revised Date: 2012-09-18 |
Medline Journal Info:
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Nlm Unique ID: 101197107 Medline TA: Stem Cells Dev Country: United States |
Other Details:
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Languages: eng Pagination: 795-807 Citation Subset: IM |
Affiliation:
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Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation / genetics Cell Line Female Fibroblasts / cytology, metabolism* Gammaretrovirus / genetics, metabolism Genetic Vectors / genetics, metabolism Humans Induced Pluripotent Stem Cells* / cytology, metabolism Kruppel-Like Transcription Factors / genetics, metabolism Macaca nemestrina / genetics*, metabolism Male Mice Microarray Analysis Nuclear Reprogramming* / genetics Octamer Transcription Factor-3 / genetics, metabolism Proto-Oncogene Proteins c-myc / genetics, metabolism Regenerative Medicine Reverse Transcriptase Polymerase Chain Reaction Risk SOXB1 Transcription Factors / genetics, metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/GKLF protein; 0/Kruppel-Like Transcription Factors; 0/Octamer Transcription Factor-3; 0/Proto-Oncogene Proteins c-myc; 0/SOX2 protein, human; 0/SOXB1 Transcription Factors |
| Comments/Corrections | |
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