Document Detail


Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos.
MedLine Citation:
PMID:  20582321     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genetically modified mouse strains derived from embryonic stem (ES) cells have become essential tools for functional genomics and biomedical research. Large scale mutagenesis projects are producing libraries of mutant C57BL/6 (B6) ES cells to enable the functional annotation of every gene of the mouse genome. To realize the utility of these resources, efficient and accessible methods of generating mutant mice from these ES cells are necessary. Here, we describe a combination of ICR morula aggregation and a chemically-defined culture medium with widely available and accessible components for the high efficiency generation of germline transmitting chimeras from C57BL/6N ES cells. Together these methods will ease the access of the broader biomedical research community to the publicly available B6 ES cell resources.
Authors:
Marina Gertsenstein; Lauryl M J Nutter; Tammy Reid; Monica Pereira; William L Stanford; Janet Rossant; Andras Nagy
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-22
Journal Detail:
Title:  PloS one     Volume:  5     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2010  
Date Detail:
Created Date:  2010-06-28     Completed Date:  2010-09-01     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e11260     Citation Subset:  IM    
Affiliation:
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chimera*
Culture Media
Embryo, Mammalian / cytology*
Embryonic Stem Cells / cytology*
Germ Cells*
Mice
Mice, Inbred C57BL
Grant Support
ID/Acronym/Agency:
1U01DA23284/DA/NIDA NIH HHS; 5U01DA023284-2/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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