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Efficient Cell Transfection with Melamine-Based Gemini Surfactants.
MedLine Citation:
PMID:  23297813     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Gemini surfactants consisting of two melamine scaffolds connected by a n-hexyl linker, and functionalised with a 1-propylammonium polar head and a lipophilic chain having variable carbon length (from C8 to C16) were synthesised. These were then used successfully for the transfection of A549, U87MG AND BRISTOL 8 cell lines with maxGFP expressing plasmid. The transfection protocol was optimised appropriately (confluence, reagent/pcDNA ratio, compaction time and transfection time), for each cell line. Under optimised conditions, the C12 and C14 melamine gemini surfactants showed little toxicity and remarkable transfection efficiency, superior to the gold-standard Lipofectamine 2000. These reagents were also able to transfect efficiently primary DRG neurons, which are notoriously difficult to transfect. The presence of serum completely inhibited the transfection capacity of these reagents. Owing to their ready availability, straightforward synthesis, high chemical stability (even in solution), ease of use (no formulation is required), improved transfection ability and low toxicity, melamine-based gemini surfactants are very promising reagents for cellular DNA transfection.
Authors:
Matteo Zanda; Serena Perrone; Michele Usai; Paolo Lazzari; Steven J Tucker; Heather Wallace
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-8
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  -     ISSN:  1520-4812     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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