Document Detail


Efficiency and safety of a technique for drawing blood from the hamster cranial vena cava.
MedLine Citation:
PMID:  19455167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The authors evaluated the efficiency and safety of a technique for drawing blood from the cranial vena cava of the hamster (Mesocricetus auratus). They collected blood from Golden Syrian hamsters (n = 15) and from epileptic hamsters (GASH/Sal strain; n = 10) that were anesthetized with isoflurane. Four epileptic hamsters constituted a control group that underwent anesthesia but not blood collection. For blood sampling, a needle connected to a syringe was inserted at a site between 0.2 and 0.6 cm from the manubrium; the depth of insertion was 0.3-1.0 cm. Blood collection was successful in all hamsters except one, and the volume of collected blood ranged from 0.2 ml to 1.5 ml. All hamsters recovered quickly from the procedure, and none showed obvious signs of pain or stress. At necropsy (24 h after the procedure), six subjects showed small local hematomas, and eight showed vascular lacerations of limited severity. Lungs and hearts did not show any damage related to vein puncture. This relatively simple blood collection technique seems to be efficient and safe in hamsters.
Authors:
María G Picazo; Pedro J Benito; Dolores C García-Olmo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lab animal     Volume:  38     ISSN:  0093-7355     ISO Abbreviation:  Lab Anim (NY)     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-20     Completed Date:  2009-07-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417737     Medline TA:  Lab Anim (NY)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  211-6     Citation Subset:  IM    
Affiliation:
Experimental Research Unit, General University Hospital of Albacete, Albacete, Spain.
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MeSH Terms
Descriptor/Qualifier:
Anesthesia / veterinary
Anesthetics, Inhalation
Animals
Cricetinae
Female
Isoflurane
Male
Mesocricetus
Pain / etiology
Phlebotomy / adverse effects,  veterinary*
Vena Cava, Superior*
Chemical
Reg. No./Substance:
0/Anesthetics, Inhalation; 26675-46-7/Isoflurane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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