Document Detail


Efficiency of cytoplasmic delivery by non-cationic liposomes to cells in vitro: a confocal laser scanning microscopy study.
MedLine Citation:
PMID:  18590977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is necessary to understand liposomal uptake mechanisms and intracellular distribution in order to design more efficient gene (drug) carrier systems. Until now, a few studies have been carried out using confocal laser scanning microscopy (CLSM) to investigate the cellular uptake and transfection mediated with liposomes. So, by CLSM, we demonstrated that artificial virus-like envelope (AVE) vesicles labeled with rhodamine-PE (Rh-PE), carbocyanine (DiI) and carboxyfluorescein (CF) were investigated into the cytoplasm of two human cell lines, Mewo (human melanoma cell line) and HepG2 (human hepatoma cell line) cells grown in DMEM medium supplemented with different percentages (0%, 30%, and 100%) fetal calf serum (FCS). The liposome uptake was dependent on the cell line, in view that the whole process of liposomes associated with cells (uptake) is a two-step process involving binding and endocytosis. Based upon the various assays used to measure cellular uptake of liposomes, we conclude the efficacy of cytoplasmic delivery by AVE-liposomes to cells in culture.
Authors:
Mohsen M Mady; M M Ghannam; W A Khalil; R Müller; Alfred Fahr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-30
Journal Detail:
Title:  Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)     Volume:  25     ISSN:  1120-1797     ISO Abbreviation:  Phys Med     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-12     Completed Date:  2009-07-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9302888     Medline TA:  Phys Med     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  88-93     Citation Subset:  IM    
Affiliation:
Department of Biophysics, Faculty of Science, Cairo University, 12613 Giza, Egypt. dr_mmady@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cytoplasm / pathology,  physiology*
Drug Carriers / chemistry*
Endocytosis*
Humans
Liposomes / chemistry*
Liver Neoplasms / pathology,  physiopathology*
Melanoma / pathology,  physiopathology*
Microscopy, Confocal / methods*
Chemical
Reg. No./Substance:
0/Drug Carriers; 0/Liposomes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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