Document Detail

Efficiency of cytoplasmic delivery by non-cationic liposomes to cells in vitro: a confocal laser scanning microscopy study.
MedLine Citation:
PMID:  18590977     Owner:  NLM     Status:  MEDLINE    
It is necessary to understand liposomal uptake mechanisms and intracellular distribution in order to design more efficient gene (drug) carrier systems. Until now, a few studies have been carried out using confocal laser scanning microscopy (CLSM) to investigate the cellular uptake and transfection mediated with liposomes. So, by CLSM, we demonstrated that artificial virus-like envelope (AVE) vesicles labeled with rhodamine-PE (Rh-PE), carbocyanine (DiI) and carboxyfluorescein (CF) were investigated into the cytoplasm of two human cell lines, Mewo (human melanoma cell line) and HepG2 (human hepatoma cell line) cells grown in DMEM medium supplemented with different percentages (0%, 30%, and 100%) fetal calf serum (FCS). The liposome uptake was dependent on the cell line, in view that the whole process of liposomes associated with cells (uptake) is a two-step process involving binding and endocytosis. Based upon the various assays used to measure cellular uptake of liposomes, we conclude the efficacy of cytoplasmic delivery by AVE-liposomes to cells in culture.
Mohsen M Mady; M M Ghannam; W A Khalil; R Müller; Alfred Fahr
Related Documents :
23727117 - Comparison of the sensitivities of three commercial assays for detection of the high ri...
18773947 - Liposomal nanomedicines as anticancer therapeutics: beyond targeting tumor cells.
18183127 - Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes.
16106367 - Cellular disorders induced by high magnetic fields.
24460377 - Neuroprotective action of deer bone extract against glutamate or aβ1-42-induced oxidati...
23043957 - Role of matrix metalloproteinases in migration and neurotrophic properties of nasal olf...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-30
Journal Detail:
Title:  Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)     Volume:  25     ISSN:  1120-1797     ISO Abbreviation:  Phys Med     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-12     Completed Date:  2009-07-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9302888     Medline TA:  Phys Med     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  88-93     Citation Subset:  IM    
Department of Biophysics, Faculty of Science, Cairo University, 12613 Giza, Egypt.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line, Tumor
Cytoplasm / pathology,  physiology*
Drug Carriers / chemistry*
Liposomes / chemistry*
Liver Neoplasms / pathology,  physiopathology*
Melanoma / pathology,  physiopathology*
Microscopy, Confocal / methods*
Reg. No./Substance:
0/Drug Carriers; 0/Liposomes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Biodosimetry using micronucleus assay in acute partial body therapeutic irradiation.
Next Document:  Role modeling excellence in clinical nursing practice.