Document Detail


Efficacy of washing with a commercial flatbed brush washer, using conventional and experimental washing agents, in reducing populations of Escherichia coli on artificially inoculated apples.
MedLine Citation:
PMID:  11271760     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Conventional and experimental washing formulations were applied with a commercial flatbed brush washer under conditions representative of commercial practice to determine their efficacy in decontaminating apples inoculated with a nonpathogenic Escherichia coli strain. Golden Delicious apples (18 kg) inoculated with E. coli were mixed with approximately 109 kg of uninoculated Fuji apples (distinctly different in appearance) in a wet dump tank containing 1,325 liters of water at 20 degrees C for 15 min. The combined apples were washed in a flatbed brush washer with the following washing solutions: water at 20 degrees C, water at 50 degrees C, 200 ppm of chlorine (pH 6.4) at 20 degrees C, 8% trisodium phosphate at 20 degrees C, 8% trisodium phosphate at 50 degrees C, 5% hydrogen peroxide at 20 degrees C, 5% hydrogen peroxide at 50 degrees C, 1% APL Kleen 245 at 50 degrees C, and two-stage washing treatments using the combination of 1% APL Kleen 245 at 20 or 50 degrees C followed by 5% hydrogen peroxide at 35 or 50 degrees C. None of the washing treatments tested under the conditions of this experiment significantly reduced the E. coli populations on the inoculated apples or in cider made from these apples, probably as a consequence of the inability of this washing system to inactivate or remove the bacterial cells in inaccessible calyx and stem areas of apples. These results are important because they demonstrate the need for new fruit washing technology that can overcome this limitation. Also, there was no significant cross-contamination of the Fuji apples in the dump tank. Significant cross-contamination of cider, made with uninoculated apples, occurred in the hammer mill and/or the press cloth when these units were not sanitized following a trial with inoculated apples.
Authors:
B A Annous; G M Sapers; A M Mattrazzo; D C Riordan
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  64     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-10-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  159-63     Citation Subset:  IM    
Affiliation:
US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA. bannous@arserrc.gov
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MeSH Terms
Descriptor/Qualifier:
Colony Count, Microbial
Disinfectants / pharmacology*
Disinfection / methods*
Escherichia coli / drug effects*,  growth & development
Food Contamination / prevention & control
Food Handling / methods
Microscopy, Electron, Scanning
Rosales / microbiology*
Treatment Outcome
Water Microbiology
Chemical
Reg. No./Substance:
0/Disinfectants

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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