| Efficacy of polyethylene glycols in University of Wisconsin preservation solutions: a study of isolated perfused rat liver. | |
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MedLine Citation:
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PMID: 16386593 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent reports argue that the performance of University of Wisconsin (UW) solution is limited by the presence of hydroxyethyl starch (HES) as an additive, since HES could be responsible for human red blood cell aggregation. We investigated the effect on rat liver preservation of replacing HES in UW solution by polyethylene glycols (PEG20 and PEG35) at two concentrations. An isolated perfused rat liver model was used. Six groups of preserved livers (n = 7 for each group) were compared to controls (nonpreserved livers, n = 7). The following preservation solutions were assayed: UW without oncotic supply, UW-HES (0.25 mmol/L), UW-PEG20 (0.03 and 0.25 mmol/L), and UW-PEG35 (0.03 and 0.25 mmol/L). After 24-hour cold storage, the livers were perfused for 120 minutes at 37 degrees C with oxygenated Krebs-Henseleit solution. During perfusion, transaminase release, portal and bile flows, and bromosulfophthalein (BSP) clearance were assessed. Results showed that the omission of oncotic supply in UW statistically increased ALT and AST release in perfusate and decreased bile and portal flows. PEG addition in UW solution, especially PEG35 at 0.25 mmol/L, effectively protected the rat liver graft from the onset of hypothermic ischemia/reperfusion damage. In conclusion, data reported here reveal that oncotic supply is essential for liver preservation and that HES can be effectively replaced by PEG in UW solution. |
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Authors:
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I Ben Mosbah; D Saidane; C Peralta; J Roselló-Catafau; H Ben Abdennebi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transplantation proceedings Volume: 37 ISSN: 0041-1345 ISO Abbreviation: Transplant. Proc. Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2006-01-02 Completed Date: 2006-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
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Languages: eng Pagination: 3948-50 Citation Subset: IM |
Affiliation:
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Departamento de Patología Experimental, Instituto de Investigaciones Biomédicas de Barcelona, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Alanine Transaminase / blood Allopurinol Animals Aspartate Aminotransferases / blood Bile / secretion Glutathione Insulin Liver / drug effects, physiology* Liver Function Tests Male Organ Preservation Solutions / pharmacology* Polyethylene Glycols / pharmacology* Portal System / drug effects, physiology Raffinose Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Organ Preservation Solutions; 0/Polyethylene Glycols; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 315-30-0/Allopurinol; 512-69-6/Raffinose; 58-61-7/Adenosine; 70-18-8/Glutathione; EC 2.6.1.1/Aspartate Aminotransferases; EC 2.6.1.2/Alanine Transaminase |
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