Document Detail

Efficacy of photocrosslinkable chitosan hydrogel containing fibroblast growth factor-2 in a rabbit model of chronic myocardial infarction.
MedLine Citation:
PMID:  15916971     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Therapeutic angiogenesis in ischemic myocardium has been shown to be an effective strategy to improve regional blood flow and myocardial function. However, no effective delivery system for growth factor administration is yet known to induce important therapeutic angiogenic responses in ischemic myocardium. MATERIALS AND METHODS: FGF-2-incorporated chitosan (FGF-2/chitosan) hydrogels were immobilized on the surface of ischemic myocardium of rabbit models of chronic myocardial infarction by UV-irradiation. After 4 weeks, cardiac functional analyses by noradrenalin challenge and histopathological analyses were performed to evaluate the efficacy of a controlled release of FGF-2 from FGF-2/chitosan hydrogel immobilized on the surface of ischemic myocardium. RESULTS: Significant improvement by application of FGF-2/chitosan hydrogels was found in systolic pressure at the left ventricle, +dp/dt maximum, and -dp/dt maximum during noradrenalin challenge at a dose of 1 microg/kg/min. Histological observations showed that a significantly larger amount of viable myocardium and CD 31 immunostained blood vessels were found in the FGF-2/chitosan hydrogel-applied group than only the chitosan-applied and control groups. CONCLUSIONS: These preliminary results indicate that the controlled release of biologically active FGF-2 molecules from FGF-2/chitosan hydrogel induces angiogenesis and possibly collateral circulation in ischemic myocardium, thereby protecting the myocardium.
Masanori Fujita; Masayuki Ishihara; Yuji Morimoto; Masafumi Simizu; Yoshio Saito; Hirofumi Yura; Takemi Matsui; Bonpei Takase; Hidemi Hattori; Yasuhiro Kanatani; Makoto Kikuchi; Tadaaki Maehara
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of surgical research     Volume:  126     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-26     Completed Date:  2005-07-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  IM    
Department of Medical Engineering, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
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MeSH Terms
Chitosan / administration & dosage*
Coronary Circulation / drug effects
Disease Models, Animal
Fibroblast Growth Factor 2 / administration & dosage*
Hydrogels / administration & dosage*
Myocardial Infarction / drug therapy*,  pathology,  physiopathology
Myocardium / pathology
Reg. No./Substance:
0/Hydrogels; 103107-01-3/Fibroblast Growth Factor 2; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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