Document Detail


Efficacy of a novel sphingosine kinase inhibitor in experimental Crohn's disease.
MedLine Citation:
PMID:  20151210     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: Activation of sphingosine kinase (SK) is a key response to many inflammatory processes. The present studies test the hypothesis that an orally available SK inhibitor, ABC294640, would be effective in rodent models of Crohn's disease. METHODS: Trinitrobenzene sulfonic acid (TNBS) was administered rectally to mice and rats. Rats were treated with ABC294640 orally alone or in combination with olsalazine and disease progression was monitored. RESULTS: For both rodent species, treatment with ABC294640 attenuated disease progression. Colon samples from the ABC294640-treated animals had improved histology and cytokine parameters when compared with vehicle-treated animals. The expression of SK was similarly increased in TNBS-treated animals and in human colon tissue specimens from inflammatory bowel disease patients relative to normal, control patients. CONCLUSIONS: Sphingosine kinase may be a critical mediator of colonic damage during intestinal inflammation, and pharmacologic inhibitors of this enzyme may prove useful in the treatment of Crohn's disease.
Authors:
Lynn W Maines; Leo R Fitzpatrick; Cecelia L Green; Yan Zhuang; Charles D Smith
Publication Detail:
Type:  Journal Article     Date:  2010-02-12
Journal Detail:
Title:  Inflammopharmacology     Volume:  18     ISSN:  1568-5608     ISO Abbreviation:  Inflammopharmacology     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-15     Completed Date:  2010-09-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9112626     Medline TA:  Inflammopharmacology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  73-85     Citation Subset:  IM    
Affiliation:
Apogee Biotechnology Corporation, Hershey Center for Applied Research, 1214 Research Blvd, Suite 1016, Hummelstown, PA 17036, USA. LWMaines@apogee-biotech.com
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MeSH Terms
Descriptor/Qualifier:
Adamantane / administration & dosage,  analogs & derivatives,  pharmacology,  therapeutic use
Aminosalicylic Acids / administration & dosage,  pharmacology,  therapeutic use
Animals
Body Weight / drug effects
Colon / drug effects,  enzymology,  metabolism,  pathology
Crohn Disease / chemically induced*,  drug therapy*,  metabolism,  pathology
Disease Models, Animal
Drug Therapy, Combination
Enzyme Inhibitors / pharmacology,  therapeutic use*
Epithelial Cells / enzymology,  metabolism
Female
Gastrointestinal Agents / administration & dosage,  pharmacology,  therapeutic use
Humans
Interleukin-1beta / metabolism
Leukocytes / enzymology,  metabolism
Male
Mice
Mice, Inbred C57BL
Neutrophils / enzymology,  pathology
Peroxidase / metabolism
Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors*,  metabolism
Prednisolone / administration & dosage,  pharmacology,  therapeutic use
Pyridines / administration & dosage,  pharmacology,  therapeutic use
Rats
Rats, Sprague-Dawley
Treatment Outcome
Trinitrobenzenesulfonic Acid / administration & dosage,  pharmacology
Tumor Necrosis Factor-alpha / metabolism
Chemical
Reg. No./Substance:
0/3-(4-chlorophenyl)-adamantane-1-carboxylic acid (pyridin-4-ylmethyl)amide; 0/Aminosalicylic Acids; 0/Enzyme Inhibitors; 0/Gastrointestinal Agents; 0/Interleukin-1beta; 0/Pyridines; 0/Tumor Necrosis Factor-alpha; 15722-48-2/olsalazine; 2508-19-2/Trinitrobenzenesulfonic Acid; 281-23-2/Adamantane; 50-24-8/Prednisolone; EC 1.11.1.7/Peroxidase; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.-/sphingosine kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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