| Efficacy of in vitro treatment of chronic myelogenous leukemia cell line, K562 cells, using 4-hydroperoxycyclophosphamide, alpha-interferon and gamma-interferon. | |
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MedLine Citation:
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PMID: 8703367 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Alpha-interferon (alpha-IFN) and gamma-interferon (gamma-IFN) are highly effective biologic agents in the treatment of chronic myelogenous leukemia (CML). 4-hydroperoxycyclophosphamide (4-HC) is the most commonly used chemotherapeutic drug when purging bone marrow contaminated with malignant cells. To investigate the efficacy of alpha-IFN, gamma-IFN and 4-HC as purging agents in CML patients, K562 cells, a human CML cell line, were treated in vitro with these drugs singly or in combination. The cytotoxic effect was evaluated by MTT assay. Data were analyzed for synergism by the median effect principle of Chou and Talalay. Using Southern blot analysis, the effect of purging on the suppression of bcr gene rearrangement was also evaluated. Alpha-IFN, gamma-IFN and 4-HC all showed good concentration-dependent cytotoxic effect. Median cytotoxic doses of alpha-IFN gamma-IFN and 4-HC were 0.03 microM 0.20 microM and 1.97 microM respectively, and molar ratios of median doses of alpha-IFN to 4-HC, gamma-IFN to 4-HC and alpha-IFN to gamma-IFN were 1:80, 1:10 and 1:8, respectively. The combination treatment of alpha-IFN and 4-HC, and gamma-IFN and 4-HC showed significant synergistic cytotoxic activities, but combination of alpha-IFN and gamma-IFN was antagonistic. Status of bcr gene rearrangements in K562 cells was not changed after the treatments. Our results appear to provide an efficient method for removing CML cells from the bone marrow of CML patients. |
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Authors:
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J T Lee; S Park; J H Lee; B K Kim; N K Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of Korean medical science Volume: 11 ISSN: 1011-8934 ISO Abbreviation: J. Korean Med. Sci. Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-09-10 Completed Date: 1996-09-10 Revised Date: 2011-03-16 |
Medline Journal Info:
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Nlm Unique ID: 8703518 Medline TA: J Korean Med Sci Country: KOREA |
Other Details:
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Languages: eng Pagination: 26-32 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, Seoul National University College of Medicine, Korea. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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therapeutic use Antineoplastic Combined Chemotherapy Protocols / therapeutic use* Blotting, Southern Cyclophosphamide / analogs & derivatives, therapeutic use Drug Antagonism Drug Screening Assays, Antitumor Drug Synergism Humans Interferon-alpha / therapeutic use Interferon-gamma / therapeutic use Leukemia, Myelogenous, Chronic, BCR-ABL Positive / drug therapy* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Interferon-alpha; 39800-16-3/perfosfamide; 50-18-0/Cyclophosphamide; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Korean Med Sci Journal ID (pmc): jkms ISSN: 1011-8934 ISSN: 1598-6357 Publisher: Korean Academy of Medical Sciences |
Article Information Download PDF  Print publication date: Month: 2 Year: 1996 Volume: 11 Issue: 1 First Page: 26 Last Page: 32 ID: 3053917 PubMed Id: 8703367 |
| Efficacy of in vitro treatment of chronic myelogenous leukemia cell line, K562 cells, using 4-hydroperoxycyclophosphamide, alpha-interferon and gamma-interferon. | |
| J. T. Lee | |
| S. Park | |
| J. H. Lee | |
| B. K. Kim | |
| N. K. Kim | |
| Department of Internal Medicine, Seoul National University College of Medicine, Korea. |
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