Document Detail

Efficacy of chemical dosing methods for isolating nontuberculous mycobacteria from water supplies of dialysis centers.
MedLine Citation:
PMID:  3415237     Owner:  NLM     Status:  MEDLINE    
Investigations of nontuberculous mycobacterium (NTM) infections associated with various environmental sources have been hampered by the lack of adequate techniques for selective isolation of these organisms from environmental fluids. This study compared chemical dosing techniques for recovery of NTM from water samples collected from 115 randomly selected dialysis centers. Cell suspensions of NTM group II and IV isolates and gram-negative bacteria were exposed to solutions containing sodium hypochlorite (0.2 micrograms/ml of free available chlorine), formaldehyde (1, 0.75, or 0.5%), oxalic acid (1.25%), cetylpyridinium chloride (25 micrograms/ml), or cetyltrimethylammonium bromide (100 micrograms/ml). Results of standard membrane filtration assays with laboratory test strains and water samples from dialysis centers showed that 5 min of exposure to 1% formaldehyde effectively reduced gram-negative bacterial populations and allowed increased recovery of NTM in environmental fluids containing mixed microbial populations.
L A Carson; L B Cusick; L A Bland; M S Favero
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  54     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-10-11     Completed Date:  1988-10-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1756-60     Citation Subset:  IM    
Nosocomial Infections Laboratory Branch, Centers for Disease Control, Atlanta, Georgia 30333.
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MeSH Terms
Cetrimonium Compounds / pharmacology
Cetylpyridinium / pharmacology
Formaldehyde / pharmacology
Gram-Negative Bacteria / drug effects
Mycobacterium / isolation & purification*
Oxalates / pharmacology
Oxalic Acid
Random Allocation
Renal Dialysis*
Sodium Hypochlorite / pharmacology
Water Microbiology*
Water Supply
Reg. No./Substance:
0/Cetrimonium Compounds; 0/Oxalates; 144-62-7/Oxalic Acid; 50-00-0/Formaldehyde; 6899-10-1/cetrimonium; 7681-52-9/Sodium Hypochlorite; 7773-52-6/Cetylpyridinium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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