Document Detail


Efficacy of ultraviolet light exposure against survival of Listeria monocytogenes on conveyor belts.
MedLine Citation:
PMID:  20113207     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Listeria monocytogenes has been repeatedly isolated from foods and food-processing facilities including food contact surfaces such as conveyor belts (CB). CBs are often difficult to clean and require rigorous sanitation programs for decontamination. Ultraviolet (UV) light has exhibited microbicidal properties on food contact surfaces and this study was conducted to determine the efficacy of UV against L. monocytogenes on CB made of different materials. A four-strain cocktail of L. monocytogenes (serotypes 3A, 4A, 4B, and 4C) was made to give a suspension of approximately 10(7) CFU/mL. CBs made from four different types of materials, (1) Ropanyl DM 8/2 A2 + 04 (belt 1), (2) Volta FRMW-3.0 (belt 2), (3) Volta FRMB-3.0 (belt 3), and (4) Ropanyl DM (belt 4), were inoculated with 1 mL of the four-strain cocktail (approximately 10(7) CFU/mL) of the bacterial suspension. CBs were treated with UV light (254 nm) for 1 and 3 sec at 5.53 and 5.95 mW/cm(2). Three replications of the experiments were conducted. Two-way analysis of variance of survival populations of L. monocytogenes showed that bacterial counts were significantly reduced (p < 0.05) on all belt types irrespective of UV light intensities and times of exposure. L. monocytogenes populations were reduced (p < 0.05) to below detection limits on belts 1, 2, and 3 after exposure to 5.95 mW/cm(2) UV light intensity for 3 sec. L. monocytogenes-inoculated CBs that were exposed to 5.53 mW/cm(2) showed higher (p < 0.05) survival populations of L. monocytogenes compared with 5.95 mW/cm(2) on all the four CBs. Belt 4 showed survival populations of L. monocytogenes ranging from 1.42 to 1.73 log(10) CFU/cm(2) after UV light treatment for 1 and 3 sec. UV light can be effectively used to reduce L. monocytogenes contamination on CBs.
Authors:
Amit Morey; Shelly R McKee; James S Dickson; Manpreet Singh
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Foodborne pathogens and disease     Volume:  7     ISSN:  1556-7125     ISO Abbreviation:  Foodborne Pathog. Dis.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-03     Completed Date:  2010-08-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120121     Medline TA:  Foodborne Pathog Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  737-40     Citation Subset:  IM    
Affiliation:
Department of Poultry Science, Auburn University, Auburn, Alabama 36849, USA.
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MeSH Terms
Descriptor/Qualifier:
Colony Count, Microbial
Disinfection / methods*
Elastomers
Equipment Contamination / prevention & control*
Food-Processing Industry / instrumentation,  methods
Foodborne Diseases / prevention & control
Listeria monocytogenes / isolation & purification,  radiation effects*
Microbial Viability / radiation effects*
Polyurethanes
Surface Properties
Time Factors
Ultraviolet Rays*
Chemical
Reg. No./Substance:
0/Elastomers; 0/Polyurethanes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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