Document Detail

Efficacy of the Ovsynch protocol for synchronization of ovulation and fixed-time artificial insemination in Murrah buffaloes (Bubalus bubalis).
MedLine Citation:
PMID:  16125551     Owner:  NLM     Status:  MEDLINE    
Two experiments were conducted to assess the timing and synchrony of ovulation, plasma LH concentrations, and pregnancy rate in Murrah buffaloes (Bubalus bubalis) treated with the Ovsynch (GnRH-PGF(2 alpha)-GnRH) protocol. In Experiment 1, 10 non-lactating cycling buffaloes received 10 microg of a GnRH analogue i.m. (buserelin acetate) without regard to the stage of the estrous cycle (day of treatment, day 0), followed by 25mg of PGF(2 alpha) i.m. (dinoprost thromethamine) 7 days later. A second-treatment of the same GnRH analogue (10 microg, i.m.) was given 48 h after PGF(2 alpha). Ovulation was confirmed by transrectal palpation (at 2-h intervals) from the second-GnRH treatment to detection of ovulation or up to 96 h after the second-GnRH treatment. Plasma LH concentrations were determined in blood samples collected at 15-min intervals for 6h, starting at the second-GnRH treatment, and thereafter at 2-h intervals until 2h after detection of ovulation. Ovulation occurred in 9/10 buffalo (90%) 23.3+/-1.3h (mean+/-S.E.M.; range 20--32 h) after the second-GnRH treatment. Peak LH concentrations 13.5+/-3.5 ng/mL (range 3.9--40.0 ng/mL) occurred 2.1+/-0.1h (range 1.2-3.0 h) after the second-GnRH treatment. In Experiment 2, 15 lactating, cycling buffaloes were subjected to the Ovsynch protocol, with fixed-time AI 12 and 24h after the second-GnRH treatment and 75 lactating buffaloes were inseminated, approximately 12h after detection of spontaneous estrus. Pregnancy rates were 33.3% for TAI and were 30.7% for buffaloes inseminated following spontaneous estrus (P=0.84). In conclusion, the Ovsynch protocol effectively synchronized ovulation in Murrah buffaloes and resulted in conception rates (to two fixed-time inseminations) that were comparable to those achieved with a single AI after detection of spontaneous estrus.
Vijay Paul; B S Prakash
Publication Detail:
Type:  Journal Article     Date:  2005-03-02
Journal Detail:
Title:  Theriogenology     Volume:  64     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-29     Completed Date:  2005-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1049-60     Citation Subset:  IM    
Division of Dairy Cattle Physiology, National Dairy Research Institute, Karnal 132001 Haryana, India.
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MeSH Terms
Buffaloes / physiology*
Buserelin / administration & dosage*
Dinoprost / administration & dosage*
Gonadotropin-Releasing Hormone / analogs & derivatives
Insemination, Artificial / methods,  veterinary*
Luteinizing Hormone / blood
Ovulation Induction / methods,  veterinary*
Time Factors
Reg. No./Substance:
33515-09-2/Gonadotropin-Releasing Hormone; 551-11-1/Dinoprost; 57982-77-1/Buserelin; 9002-67-9/Luteinizing Hormone

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