| Efficacies of inactivated vaccines against betanodavirus in grouper larvae (Epinephelus coioides) by bath immunization. | |
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MedLine Citation:
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PMID: 18276044 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Betanodavirus is the pathogen of viral nervous necrosis (VNN) disease that has caused mass mortality among many species of marine fish at larval stage. In this study, the efficacy of inactivated betanodavirus was evaluated by bath-immunization and bath-challenge of orange-spotted grouper (Epinephelus coioides) at early larval stage. Two kinds of chemicals were used for inactivation of the virus, and the relative percent survival (RPS) values of 0.4mM binary ethylenimine (BEI)-inactivated vaccine was revealed to be 79-95, higher than that of 0.1-0.2% formalin-inactivated vaccines (39-43). Three lengths of bath immunization time were tested, and 20 min immersion of BEI-inactivated betanodavirus at a concentration of 10(6)TICD(50)/ml was sufficient to induce high protection (RPS > 75). Protection of the BEI-inactivated vaccine was evaluated at different time post immunization, and the peak of protection was observed 30 days post vaccination, and retained for at least 3 months. The efficacies of formalin-inactivated vaccines with or without encapsulation were compared, and the result revealed that the efficacy of formalin-inactivated vaccine could be significantly improved by nano-encapsulation (RPS = 85). All these data strongly suggested that bath immunization with nano-encapsulated formalin-inactivated or BEI-inactivated betanodavirus vaccines is an effective strategy to protect grouper larvae against VNN. |
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Authors:
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Yu-Hsuan Kai; Shau-Chi Chi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-13 |
Journal Detail:
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Title: Vaccine Volume: 26 ISSN: 0264-410X ISO Abbreviation: Vaccine Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-04 Completed Date: 2008-05-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1450-7 Citation Subset: IM |
Affiliation:
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Institute of Zoology, National Taiwan University, Taipei, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Viral / immunology Dose-Response Relationship, Immunologic Drug Compounding Fish Diseases / immunology*, prevention & control, virology* Fishes / physiology* Immunization / methods*, veterinary* Larva Nodaviridae / immunology* Reverse Transcriptase Polymerase Chain Reaction Vaccines, Inactivated Viral Vaccines / administration & dosage, immunology*, therapeutic use* Virus Diseases / immunology*, prevention & control, veterinary* Virus Inactivation |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Viral; 0/Vaccines, Inactivated; 0/Viral Vaccines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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