Document Detail


Effects of warm acclimation on Na+,K+-ATPase alpha-subunit expression in chloride cells of Antarctic fish.
MedLine Citation:
PMID:  15912523     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The teleosts Trematomus bernacchii thrive in southern oceanic waters with temperatures below 0 degrees C. These fish have serum osmolalities almost double those found in fish of temperate waters, thereby lowering their serum's freezing point and the energy needed for ionic homeostasis. Upon warm acclimation to 4 degrees C, T. bernacchii decrease their serum osmolality and increase the Na+,K+-ATPase activity in their gills. Na+,K+-ATPase alpha1-, alpha2-, and alpha3-subunit isoforms are expressed in the gills of T. bernacchii and it is thought that Na+,K+-ATPase subunit composition in chloride cells changes with warm acclimation. Using immunohistochemistry, we compared the number of chloride cells expressing various alpha-isoforms of the Na+,K+-ATPase in the gills of cold- and warm-acclimated T. bernacchii. We found no change in the number of alpha2- or alpha3-immunopositive cells in warm-acclimated fish gills or in the number of cells immunopositive for the Na+,K+,2Cl- cotransporter. However, the number of pan-alpha-immunopositive (recognizing all three alpha-isoforms) and alpha1-immunopositive cells both increased in warm-acclimated fish. This suggests that changes in the number of alpha1-isoform-expressing chloride cells could contribute to the increased Na+,K+-ATPase activity that occurs with warm-acclimation.
Authors:
Philip R Brauer; Jennifer N Sanmann; David H Petzel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology     Volume:  285     ISSN:  1552-4884     ISO Abbreviation:  -     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-27     Completed Date:  2005-09-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101234285     Medline TA:  Anat Rec A Discov Mol Cell Evol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  600-9     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2005 Wiley-Liss, Inc.
Affiliation:
Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska 68178, USA. prbrauer@creighton.edu
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MeSH Terms
Descriptor/Qualifier:
Acclimatization / physiology*
Animals
Antarctic Regions
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Fishes / physiology*
Fluorescent Antibody Technique, Indirect
Gills / anatomy & histology,  enzymology*
Hot Temperature*
Isoenzymes
Osmolar Concentration
Sodium-Potassium-Exchanging ATPase / analysis,  metabolism*
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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