| Effects of vitamin B1 (thiamine) deficiency in lake trout (Salvelinus namaycush) alevins at hatching stage. | |
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MedLine Citation:
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PMID: 19576291 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objectives of this study were to examine the relationship between thiamine concentrations in unfertilized eggs and yolksac individuals of lake trout (Salvelinus namaycush), along with any associated histopathological changes in the tissues of alevins at the hatching stage. We address these questions in a lake trout population from different spawning grounds of Lake Michigan (North and South), known for compromised survival due to early mortality syndrome (EMS). However, a dichotomous forage base of lake trout spawning stocks, with a dietary thiaminase-rich alewife in the North, and dietary low-thiaminase round goby in the South, provides the basis for the assumption that different diets may lead to differences in severity of EMS between different stocks. Lake trout eggs of 18 females were collected and fertilized individually with the sperm of several males. The eggs, eyed embryos and newly-hatched alevins were sampled to examine thiamine utilization during embryogenesis. Progenies of females with low (< 0.73 nmol/g) and high (> 0.85 nmol/g) levels of thiamine were chosen for histological studies. The obtained results showed that total thiamine levels in the body and yolk of eyed embryos and alevins at hatching were influenced by thiamine levels of unfertilized eggs and it decreased during embryogenesis (to 51% in eyed embryos and 28% in newly-hatched alevins in comparison to unfertilized eggs). The survival of lake trout until hatching stage does not correlate with the thiamine level, however it was affected by collection site and was significantly higher in fish from the South site (Julian's Reef). At the hatching stage, no pathological changes were observed in the brain, olfactory lobe, retina or liver in embryos regardless of thiamine concentrations in unfertilized eggs. It has been concluded that an enhanced thiamine requirement for the fast muscle mass growth near the swim-up stage is responsible for overt and histopathological signs of EMS. Current study confirms earlier findings that lake trout suffering from EMS can be successfully treated by immersion in thiamine solution as late as at the swim-up stage. |
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Authors:
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Marta Jaroszewska; Bong-Joo Lee; Konrad Dabrowski; Sergiusz Czesny; Jacques Rinchard; Paulina Trzeciak; Bogdana Wilczyńska |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-07-01 |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Volume: 154 ISSN: 1531-4332 ISO Abbreviation: Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-07-28 Completed Date: 2009-08-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806096 Medline TA: Comp Biochem Physiol A Mol Integr Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 255-62 Citation Subset: IM |
Affiliation:
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School of Environment and Natural Resources, The Ohio State University, Columbus, OH 43210, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / embryology, pathology Eye / embryology, pathology Female Fish Diseases / embryology, metabolism, pathology* Great Lakes Region Liver / embryology, pathology Liver Glycogen / analysis Nutritional Status* Ovum / chemistry Syndrome Thiamine / analysis* Thiamine Deficiency / embryology, mortality, pathology, veterinary* Thiamine Monophosphate / analysis Thiamine Pyrophosphate / analysis Trout / embryology, growth & development, metabolism* Yolk Sac / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Liver Glycogen; 154-87-0/Thiamine Pyrophosphate; 532-40-1/Thiamine Monophosphate; 59-43-8/Thiamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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