Document Detail


Effects of vasoactive intestinal polypeptide microinjected into the nucleus tractus solitarius on jejunal electrolytes absorption in rats.
MedLine Citation:
PMID:  15627496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been shown that vasoactive intestinal polypeptide (VIP) injected into the nucleus tractus solitarius inhibits alanine absorption across the jejunum. The aim of the present study was to investigate the effects of VIP injection into the nucleus tractus solitarius on jejunal absorption of electrolytes in the rat. Fifty-three Wistar rats were submitted to midline laparotomy to expose and isolate 20 cm of jejunal loop and to perform a subdiaphragmatic troncular vagotomy. Saline or VIP (10 pg 100 nl(-1)) was injected into the rostral nucleus tractus solitarius using a stereotaxic instrument. Tyrode solution, pH 8, containing twice glucose, sodium and potassium concentration was infused (0.5 ml min(-1)) into the jejunal loop. Samples were taken at 10-min intervals during the 40-min-experiment. Injection of VIP into the nucleus tractus solitarius increased jejunal potassium absorption. Moreover, VIP associated with vagotomy resulted in inhibition of jejunal potassium absorption by VIP alone at 40 min after perfusion (5.99 +/- 0.74 vs. 9.83 +/- 0.57 microM). There was no change in jejunal sodium absorption in any of the experimental groups. VIP had a modulatory action on jejunal potassium absorption when injected into the nucleus tractus solitarius.
Authors:
Alvaro D C V Machado; Andréa S Haibara; Andy Petroianu; Elizabeth L Borges
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-11-24
Journal Detail:
Title:  Neuropeptides     Volume:  39     ISSN:  0143-4179     ISO Abbreviation:  Neuropeptides     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-03     Completed Date:  2005-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8103156     Medline TA:  Neuropeptides     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  15-9     Citation Subset:  IM    
Affiliation:
Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cerebrospinal Fluid
Diuretics, Osmotic / pharmacology
Intestinal Absorption / drug effects*
Jejunum / innervation,  metabolism*
Male
Mannitol / pharmacology
Microinjections
Potassium / pharmacokinetics
Rats
Rats, Wistar
Sodium / pharmacokinetics
Sodium Chloride / pharmacology
Solitary Nucleus / drug effects*,  physiology*
Sympathectomy
Vagotomy
Vasoactive Intestinal Peptide / pharmacology*
Chemical
Reg. No./Substance:
0/Diuretics, Osmotic; 37221-79-7/Vasoactive Intestinal Peptide; 69-65-8/Mannitol; 7440-09-7/Potassium; 7440-23-5/Sodium; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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