Document Detail

Effects of various oxidants and antioxidants on the p38-MAPK signalling pathway in the perfused amphibian heart.
MedLine Citation:
PMID:  16710743     Owner:  NLM     Status:  MEDLINE    
We investigated the effects of different antioxidants such as L-ascorbic acid, catalase, and superoxide dismutase (SOD), on the p38-MAPK activation induced by oxidative stress in the isolated perfused amphibian heart. Oxidative stress was exemplified by perfusing hearts with 30 microM H(2)O(2) for 5 min or with the enzymatic system of xanthine/xanthine oxidase (200 microM/10 mU/ml, respectively) for 10 min. H(2)O(2)-induced activation of p38-MAPK (7.04 +/- 0.20-fold relative to control values) was totally attenuated by L-ascorbic acid (100 microM) or catalase (150 U/ml). These results were confirmed by immunohistochemical studies in which the phosphorylated form of p38-MAPK was localised in the perinuclear region and dispersedly in the cytoplasm of the ventricular cells during H(2)O(2) treatment, a pattern that was abolished by catalase or L-ascorbic acid. p38-MAPK was also activated (2.34 +/- 0.17-fold) by perfusing amphibian hearts with the reactive oxygen species (ROS)-generating system of xanthine/xanthine oxidase and this activation sustained in the presence of 150 U/ml catalase (2.16 +/- 0.26-fold), 50 U/ml SOD (2.02 +/- 0.07) or 100 microM L-ascorbic acid (2.18 +/- 0.10), but was suppressed by the combination of 150 U/ml catalase and 50 U/ml SOD. Finally, our studies showed that xanthine/xanthine oxidase induced the phosphorylation of the potent p38-MAPK substrates MAPKAPK2 (3.14 +/- 0.27-fold) and HSP27 (5.32 +/- 0.83-fold), which are implicated in cell protection, and this activation was reduced by the simultaneous use of catalase and SOD.
Catherine Gaitanaki; Maria Papatriantafyllou; Konstantina Stathopoulou; Isidoros Beis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-20
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  291     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-12     Completed Date:  2006-12-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  107-17     Citation Subset:  IM    
Department of Animal & Human Physiology, School of Biology, Faculty of Sciences, University of Athens, Panepistimioupolis, Athens, 157 84, Greece.
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MeSH Terms
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology
Catalase / pharmacology
Heart / drug effects*
Hydrogen Peroxide / pharmacology
Intracellular Signaling Peptides and Proteins
Myocardium / enzymology,  metabolism*
Oxidants / pharmacology*
Phosphorylation / drug effects
Protein-Serine-Threonine Kinases / metabolism
Signal Transduction / drug effects*
Xanthine / metabolism
Xanthine Oxidase / metabolism
p38 Mitogen-Activated Protein Kinases / metabolism*
Reg. No./Substance:
0/Antioxidants; 0/Intracellular Signaling Peptides and Proteins; 0/Oxidants; 50-81-7/Ascorbic Acid; 69-89-6/Xanthine; 7722-84-1/Hydrogen Peroxide; EC; EC Oxidase; EC 2.7.1.-/MAP-kinase-activated kinase 2; EC Kinases; EC Mitogen-Activated Protein Kinases

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