Document Detail

Effects of various forms of surfactant protein C on tidal volume in ventilated immature newborn rabbits.
MedLine Citation:
PMID:  12433871     Owner:  NLM     Status:  MEDLINE    
Surfactant protein (SP)-C is characterized by alpha-helix structure and palmitoyl groups attached to two cysteine residues. We examined the function of palmitoylation and dimerization in promotion of tidal volume in immature newborn rabbits. Reconstituted surfactants were made from a mixture of synthetic phospholipids and porcine SP-B (basic mixture) by adding various forms of SP-Cs: normal SP-C isolated from porcine lungs and monomeric or dimeric forms of SP-C. These latter two were isolated from patients with pulmonary alveolar proteinosis and were less palmitoylated. Animals were ventilated at an inspiratory pressure of 25 cmH2O. Median tidal volumes were <2 ml/kg in nontreated controls, 7.7 ml/kg in animals receiving the basic mixture without SP-C, and >18 ml/kg in animals treated with reconstituted surfactants containing 3% normal or 2% dimeric SP-C (P < 0.05 vs. basic mixture). The physiological effect of basic mixture was not improved by monomeric SP-C. We conclude that palmitoyl groups are important for the physiological effects of SP-C and that the dimeric form also improves physiological effects.
Katsumi Tashiro; Keisuke Ohta; Xiaoguang Cui; Kazuo Nishizuka; Ken Yamamoto; Tomoharu Konzaki; Tsutomu Kobayashi; Yasuhiro Suzuki
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-10-25
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  94     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-10     Completed Date:  2003-10-29     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1519-26     Citation Subset:  IM    
Department of Anesthesiology and Intensive Care Medicine, Graduate School of Medicine, Kanazawa University, Kanazawa 920-8641, Japan.
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MeSH Terms
Animals, Newborn / physiology*
Palmitic Acid / metabolism
Protein Structure, Secondary
Pulmonary Surfactant-Associated Protein C / chemistry,  pharmacology*
Tidal Volume / drug effects*
Reg. No./Substance:
0/Pulmonary Surfactant-Associated Protein C; 57-10-3/Palmitic Acid

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