| Effects of various fatty acid amendments on a microbial digester community in batch culture. | |
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MedLine Citation:
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PMID: 21071199 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Since biogas production is becoming increasingly important the understanding of anaerobic digestion processes is fundamental. However, large-scale digesters often lack online sensor equipment to monitor key parameters. Furthermore the possibility to selectively change fermenting parameter settings in order to investigate methane output or microbial changes is limited. In the present study we examined the possibility to investigate the microbial community of a large scale (750,000L) digester within a laboratory small-scale approach. We studied the short-term response of the downscaled communities on various fatty acids and its effects on gas production and compared it with data from the original digester sludge. Even high loads of formic acid led to distinct methane formation, whereas high concentrations of other acids (acetic, butyric, propionic acid) caused a marked inhibition of methanogenesis coupled with an increase in hydrogen concentration. Molecular microbial techniques (DGGE/quantitative real-time-PCR) were used to monitor the microbial community changes which were related to data from GC and HPLC analysis. DGGE band patterns showed that the same microorganisms which were already dominant in the original digester re-established again in the lab-scale experiment. Very few microorganisms dominated the whole fermenting process and species diversity was not easily influenced by moderate varying fatty acid amendments -Methanoculleus thermophilus being the most abundant species throughout the variants. MCR-copy number determined via quantitative real-time-PCR - turned out to be a reliable parameter for quantification of methanogens, even in a very complex matrix like fermenter sludge. Generally the downscaled batch approach was shown to be appropriate to investigate microbial communities from large-scale digesters. |
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Authors:
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Andreas O Wagner; Cornelia Malin; Philipp Lins; Paul Illmer |
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Publication Detail:
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Type: Journal Article Date: 2010-11-10 |
Journal Detail:
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Title: Waste management (New York, N.Y.) Volume: 31 ISSN: 1879-2456 ISO Abbreviation: Waste Manag Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-01-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9884362 Medline TA: Waste Manag Country: United States |
Other Details:
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Languages: eng Pagination: 431-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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University of Innsbruck, Institute of Microbiology, Technikerstr. 25, A-6020 Innsbruck, Austria. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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