| Effects of varicocele upon the expression of apoptosis-related proteins. | |
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MedLine Citation:
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PMID: 20629644 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Varicocele-associated apoptosis has been recognised as a cause of male infertility. Thus, we assessed the expression of somatic apoptosis-related proteins (the typical protein-dependent apoptosis markers) in ejaculated sperm plasma from both patients with varicocele and normal donors. We evaluated the relationships between certain apoptosis-related proteins and normal semen quality. Semen samples were obtained from 25 patients with varicocele and from 10 normal fertile controls. These samples were compared using computer-assisted semen analysis for motion parameters and manual analysis for morphology, and were also assayed for apoptosis-related protein activation including caspase-3, poly-ACP-ribose polymerase (PARP), the Bcl-2 family (Bcl-2, Bak) and p53 by means of immunoblot analysis. PARP, Bak and p53 were expressed substantially more in the sperm cells of the varicocele group when compared with the normal group (P < 0.05). The expression of caspase-3 and Bcl-2 did not appear to differ between these two study groups. An increased expression of PARP, Bak and p53 for varicocele-afflicted individuals indicated an increased participation by these agents in the regulating of apoptosis in the ejaculated semen from patients with varicocele, suggesting that certain protein-development apoptotic mechanisms might originate in the cytoplasmic droplet or within mitochondria of spermatocytes and then might function within the nucleus of the cell. |
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Authors:
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F-W Chang; G-H Sun; Y-Y Cheng; I-C Chen; H-H Chien; G-J Wu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Andrologia Volume: 42 ISSN: 1439-0272 ISO Abbreviation: Andrologia Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-15 Completed Date: 2010-10-18 Revised Date: 2011-12-20 |
Medline Journal Info:
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Nlm Unique ID: 0423506 Medline TA: Andrologia Country: Germany |
Other Details:
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Languages: eng Pagination: 225-30 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Reproductive Medicine Center, Taipei, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Apoptosis / physiology* Caspase 3 / biosynthesis Ejaculation / physiology Gene Expression Humans Infertility, Male / metabolism Male Poly(ADP-ribose) Polymerases / biosynthesis Proto-Oncogene Proteins c-bcl-2 / biosynthesis Semen Analysis Spermatozoa / metabolism* Tumor Suppressor Protein p53 / biosynthesis Varicocele / physiopathology* bcl-2 Homologous Antagonist-Killer Protein / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/BAK1 protein, human; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 0/bcl-2 Homologous Antagonist-Killer Protein; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3 |
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