Document Detail


Effects of urea and acetic acid on the heme axial ligation structure of ferric myoglobin at very acidic pH.
MedLine Citation:
PMID:  19622342     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The heme iron coordination of ferric myoglobin (Mb) in the presence of 9.0M urea and 8.0M acetic acid at acidic pH values has been probed by electronic absorption, magnetic circular dichroism and resonance Raman spectroscopic techniques. Unlike Mb at pH 2.0, where heme is not released from the protein despite the acid denaturation and the loss of the axial ligand, upon increasing the concentration of either urea or acetic acid, a spin state change is observed, and a novel, non-native six-coordinated high-spin species prevails, where heme is released from the protein.
Authors:
Enrica Droghetti; Suganya Sumithran; Masanori Sono; Marián Antalík; Milan Fedurco; John H Dawson; Giulietta Smulevich
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-07-19
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  489     ISSN:  1096-0384     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-14     Completed Date:  2009-09-29     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  68-75     Citation Subset:  IM    
Affiliation:
Dipartimento di Chimica, Università di Firenze, Via della Lastruccia 3, I-50019 Sesto Fiorentino (FI), Italy.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / chemistry*
Animals
Cattle
Heme / chemistry*
Horses
Hydrogen-Ion Concentration
Iron / chemistry*
Molecular Structure
Myoglobin / chemistry*
Protein Denaturation
Urea / chemistry*
Grant Support
ID/Acronym/Agency:
GM 26730/GM/NIGMS NIH HHS; R01 GM026730-22/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Myoglobin; 14875-96-8/Heme; 57-13-6/Urea; 64-19-7/Acetic Acid; 7439-89-6/Iron
Comments/Corrections

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