Document Detail

Effects of unsaturated fatty acids on calcium-activated potassium current in gastric myocytes of guinea pigs.
MedLine Citation:
PMID:  15655819     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the effects of exogenous unsaturated fatty acids on calcium-activated potassium current (I(K(Ca))) in gastric antral circular myocytes of guinea pigs. METHODS: Gastric myocytes were isolated by collagenase from the antral circular layer of guinea pig stomach. The whole-cell patch clamp technique was used to record I(K(Ca)) in the isolated single smooth muscle cells with or without different concentrations of arachidonic acid (AA), linoleic acid (LA), and oleic acid (OA). RESULTS: AA at concentrations of 2,5 and 10 micromol/L markedly increased I(K(ca)) in a dose-dependent manner. LA at concentrations of 5, 10 and 20 micromol/L also enhanced I(K(Ca)) in a dose-dependent manner. The increasing potency of AA, LA, and oleic acid (OA) on I(K(Ca)) at the same concentration (10 micromol/L) was in the order of AA>LA>OA. AA (10 micromol/L)-induced increase of I(K(ca)) was not blocked by H-7 (10 micromol/L), an inhibitor of protein kinase C (PKC), or indomethacin (10 micromol/L), an inhibitor of the cyclooxygenase pathway, and 17-octadecynoic acid (10 micromol/L), an inhibitor of the cytochrome P450 pathway, but weakened by nordihydroguaiaretic acid (10 micromol/L), an inhibitor of the lipoxygenase pathway. CONCLUSION: Unsaturated fatty acids markedly increase I(K(ca)), and the enhancing potencies are related to the number of double bonds in the fatty acid chain. The lipoxygenase pathway of unsaturated fatty acid metabolism is involved in the unsaturated fatty acid-induced increase of I(K(ca)) in gastric antral circular myocytes of guinea pigs.
Hai-Feng Zheng; Xiang-Lan Li; Zheng-Yuan Jin; Jia-Bin Sun; Zai-Liu Li; Wen-Xie Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  11     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-18     Completed Date:  2005-03-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  672-5     Citation Subset:  IM    
Shanghai Jiaotong University School of Medicine, Shanghai 200030, China.
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MeSH Terms
Arachidonic Acid / pharmacology
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Fatty Acids, Unsaturated / pharmacology*
Guinea Pigs
Linoleic Acid / pharmacology
Membrane Potentials / drug effects
Myocytes, Smooth Muscle / drug effects*,  physiology*
Oleic Acid / pharmacology
Potassium Channels, Calcium-Activated / physiology*
Protein Kinase C / antagonists & inhibitors
Pyloric Antrum / cytology*,  physiology
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Fatty Acids, Unsaturated; 0/Potassium Channels, Calcium-Activated; 112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; 506-32-1/Arachidonic Acid; EC Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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