Document Detail


Effects of ultrasound-induced inertial cavitation on enzymatic thrombolysis.
MedLine Citation:
PMID:  20687276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cavitation induced by ultrasound enhances enzymatic fibrinolysis by increasing the transport of reactants. However, the effects of cavitation need to be fully understood before sonothrombolysis can be applied clinically. In order to understand the underlying mechanisms, we examined the effects of combining ultrasound, microbubbles and thrombolytic enzymes on thrombolysis. First, we evaluated the relations between inertial cavitation and the reduction in the weight of a blood clot. Inertial cavitation was varied by changing the amplitude and duration of the transmitted acoustic wave as well as the concentration of microbubbles used to induce cavitation. Second, we studied the combined effects of streptokinase and inertial cavitation on thrombolysis. The results show that inertial cavitation increases the weight reduction of a blood clot by up to 33.9%. With linear regression fitting, the measured differential inertial cavitation dose and the weight reduction had a correlation coefficient of 0.66. Microscopically, enzymatic thrombolysis effects manifest as multiple large cavities within the clot that are uniformly distributed on the side exposed to ultrasound. This suggests that inertial cavitation plays an important role in producing cavities, while microjetting of the microbubbles induces pits on the clot surface. These observations preliminarily demonstrate the clinical potential of sonothrombolysis. The use of the differential inertial cavitation dose as an indicator of blood clot weight loss for controlled sonothrombolysis is also possible and will be further explored.
Authors:
Yueh-Hsun Chuang; Po-Wen Cheng; Szu-Chia Chen; Jia-Ling Ruan; Pai-Chi Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Ultrasonic imaging     Volume:  32     ISSN:  0161-7346     ISO Abbreviation:  Ultrason Imaging     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-08-06     Completed Date:  2010-09-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909167     Medline TA:  Ultrason Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  81-90     Citation Subset:  IM    
Affiliation:
Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Albumins / pharmacology
Contrast Media / pharmacology*
Fibrinolysis / drug effects,  physiology*
Fibrinolytic Agents / pharmacology*
Humans
Linear Models
Microbubbles
Models, Cardiovascular
Phospholipids / pharmacology
Statistics, Nonparametric
Streptokinase / pharmacology*
Sulfur Hexafluoride / pharmacology
Thrombolytic Therapy / methods*
Thrombosis / enzymology*,  therapy*
Ultrasonic Therapy / methods*
Chemical
Reg. No./Substance:
0/Albumins; 0/Contrast Media; 0/Fibrinolytic Agents; 0/Phospholipids; 0/contrast agent BR1; 2551-62-4/Sulfur Hexafluoride; EC 3.4.-/Streptokinase

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