Document Detail

Effects of type IV collagen and laminin on the cryopreservation of human embryonic stem cells.
MedLine Citation:
PMID:  15536186     Owner:  NLM     Status:  MEDLINE    
Previous reports have indicated that extracellular matrices (ECMs) affect the developmental fate of human embryonic stem cells (hESCs). Specially, type IV collagen and laminin, which belong to a group of macromolecular proteins with a substantial proportion of ECMs, are known to influence the proliferation and differentiation of hES cells. In this study, we evaluated the effects of type IV collagen and laminin in freezing medium on the survival and differentiation rates of hES cells after slow freezing and rapid thawing. The addition of type IV collagen (1 microg/ml) to the freezing medium significantly increased the survival rate of hES cells after thawing compared with that of a control group. The spontaneous differentiation rates of groups treated with type IV collagen (1 microg/ml) or laminin (1 microg/ml) were significantly lower than those of the control group. Frozen-thawed hES cells have currently been cultured for more than 70 passages and retain key properties of hES cells such as morphological characteristics, normal karyotype, marker expression (alkaline phosphatase, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, Rex-1, and Oct-4), basement membrane-related gene expression, and the potential to differentiate into derivatives of all three germ layers. This new slow freezing method by ECM treatment is a reliable and effective cryopreservation method for pluripotent hES cells.
Sun Jong Kim; Jong Hyuk Park; Jeoung Eun Lee; Jin Mee Kim; Jung Bok Lee; Shin Yong Moon; Sung Il Roh; Chul Geun Kim; Hyun Soo Yoon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Retracted Publication    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  22     ISSN:  1066-5099     ISO Abbreviation:  Stem Cells     Publication Date:  2004  
Date Detail:
Created Date:  2004-11-10     Completed Date:  2005-04-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  950-61     Citation Subset:  IM    
Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital, Kangseo-ku, Seoul 157-280, Korea.
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MeSH Terms
Alkaline Phosphatase / biosynthesis
Antigens, CD15 / biosynthesis
Antigens, Surface
Antigens, Tumor-Associated, Carbohydrate
Basement Membrane / metabolism
Cell Culture Techniques / methods
Cell Differentiation
Cell Line
Cell Proliferation
Cell Separation
Cell Survival
Cell Transplantation
Collagen Type IV / physiology*
Cryopreservation / methods*
Culture Media / pharmacology
DNA-Binding Proteins / biosynthesis
Embryo, Mammalian / cytology*
Flow Cytometry
Gene Expression
Glycoproteins / biosynthesis
Glycosphingolipids / biosynthesis
Guanine Nucleotide Exchange Factors / biosynthesis
Laminin / metabolism,  physiology*
Mice, SCID
Octamer Transcription Factor-3
Reverse Transcriptase Polymerase Chain Reaction
Stage-Specific Embryonic Antigens
Stem Cells / cytology*
Teratoma / metabolism
Time Factors
Transcription Factors / biosynthesis
Reg. No./Substance:
0/Antigens, CD15; 0/Antigens, Surface; 0/Antigens, Tumor-Associated, Carbohydrate; 0/Collagen Type IV; 0/Culture Media; 0/DNA-Binding Proteins; 0/Glycoproteins; 0/Glycosphingolipids; 0/Guanine Nucleotide Exchange Factors; 0/Laminin; 0/Octamer Transcription Factor-3; 0/POU5F1 protein, human; 0/PREX1 protein, human; 0/Pou5f1 protein, mouse; 0/Proteoglycans; 0/Stage-Specific Embryonic Antigens; 0/TRA-1-60 antigen, human; 0/Transcription Factors; 0/stage-specific embryonic antigen-3; 0/stage-specific embryonic antigen-4; EC Phosphatase
Retraction In:
Civin CI, Gewirtz AM, Goodell MA, Hawley RG, Murphy MJ. Stem Cells. 2006 Apr;24(4):804   [PMID:  16582451 ]

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